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| Acid adaptation reduces the transport ability of Salmonella typhimurium in natural soil |
| Received:November 19, 2022 Revised:December 01, 2022 |
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| KeyWord:Salmonella;acid adaptability change;surface property;transport;XDLVO model |
| Author Name | Affiliation | E-mail | | PU Zhe | Key Lab of Eco-restoration of Reginal Contaminated Environmental(Shenyang University), Ministry of Education, Shenyang 110044, China | | | YANG Xinyao | Key Lab of Eco-restoration of Reginal Contaminated Environmental(Shenyang University), Ministry of Education, Shenyang 110044, China | xinyao_yang@qq.com | | YU Dan | Key Lab of Eco-restoration of Reginal Contaminated Environmental(Shenyang University), Ministry of Education, Shenyang 110044, China | | | ZHANG Qi | Key Lab of Eco-restoration of Reginal Contaminated Environmental(Shenyang University), Ministry of Education, Shenyang 110044, China | |
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| Abstract: |
| Based on a soil column transport experiment, this study investigated the transport mechanism of Salmonella typhimurium, which underwent adaptive changes in an acidic environment（pH=5）. The results showed that acidic environment increased the particle size and concentration of lipopolysaccharide（LPS）in the extracellular membrane of Salmonella typhimurium and decreased the concentration of extracellular polymer（EPS）. These physiological changes in the extracellular membrane structure decreased the electronegativity of bacterial Zeta potential and increase its hydrophobicity, thereby changing the transport mechanism of Salmonella. Column experiment results showed that the transport ability of Salmonella decreased after their adaptation to acid stress. The reason was that the acid adaptability reduced the electrostatic repulsion between Salmonella and the surface of the sand medium and enhanced their hydrophobic adsorption. Both mechanisms promoted the deposition of Salmonella on the sand surface. The energy barrier and the second minimum depth in the XDLVO（extended Derjaguin-Landau-Verwey-Overbeek）curve indicated that the settlement of Salmonella on the sand surface was a reversible process. The results of this study show that the changes in the surface physiological characteristics of Salmonella under acid stress would inhibit their transport in soil, thereby reducing the risk of Salmonella transmission in an acidic environment. |
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