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| Characteristics and application of chlorpyrifos-degrading endophytic bacteria from rice（Oryza sativa L.） |
| Received:November 22, 2022 Revised:December 02, 2022 |
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| KeyWord:chlorpyrifos;endophyte;degrading characteristic;colonization;rice |
| Author Name | Affiliation | E-mail | | LU Yingfei | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | MA Liya | Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China | liyama@jaas.ac.cn | | ZHANG Zhaoxian | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | | | SUN Xing | Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China | | | GE Jing | Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China | | | WU Xiangwei | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China | wxw@ahau.edu.cn | | YU Xiangyang | College of Resources and Environment, Anhui Agricultural University, Hefei 230036, China
Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China | |
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| Abstract: |
| To tap the resources of chlorpyrifos-degrading endophytic bacteria and apply these bacteria in bioremediation to improve agricultural production, a chlorpyrifos-degrading endophyte strain was isolated from the roots of rice（Oryza sativa L.） using a microbiological culture method in a contaminated factory. The characteristics and effect of the strain on chlorpyrifos degradation in rice crops were investigated. Based on the morphological characteristics of the endophyte and 16S rRNA sequence analysis, the strain was identified as Bacillus sp. and named as CP40. Bacillus sp. CP40 contained opd, which encodes an organophosphorus hydrolase. The degradation rate of chlorpyrifos was 58.4% when the gene was heterologously expressed. Optimization analysis of the degradation conditions indicated that the lowest chlorpyrifos residues were observed at an initial chlorpyrifos concentration of 10 mg·L-1, temperature of 30 ℃, and pH of 7. Strain CP40 colonized and promoted the growth and development of rice plants and removed 30.9% of chlorpyrifos from these plants. Our results suggest that endophytic bacteria can reduce chlorpyrifos residues in rice. |
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