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| Humic acid combined with chitosan regulates bacterial communities in tomato facility soil |
| Received:October 25, 2022 Revised:November 17, 2022 |
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| KeyWord:humic acid;chitosan;tomato facility;bacterial wilt;high-throughput sequencing |
| Author Name | Affiliation | E-mail | | BAO Ying | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | YAN Mengyuan | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | WU Meng | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | LI Guilong | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | LIU Kai | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
University of Chinese Academy of Sciences, Beijing 100049, China | | | LI Zhongpei | State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China
University of Chinese Academy of Sciences, Beijing 100049, China | zhpli@issas.ac.cn |
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| Abstract: |
| In order to explore more efficient green control methods for bacterial wilt in tomato facilities, we collected infected soil, added biological modifiers, humic acid and / or chitosan, and incubated it at 26 ℃ for 10 weeks. The results showed that the soil bacterial community structure was significantly different with the co-application of humic acid and chitosan（HC）compared with the control and single application treatments（P＜0.05）. Under HC treatment, the relative abundance of the bacterial wilt pathogen Ralstonia spp. was significantly decreased, while the relative abundance of beneficial microorganisms, Streptomyces spp., Kitasatospora spp., Catenulispora spp., and Terracidiphilus spp., was significantly increased（P＜0.05）. The co-occurrence network results showed that the network connectivity, clustering coefficient, and average degree of HC subnetworks were significantly improved（P＜0.05）and there were direct and indirect inhibitory effects between beneficial microorganisms and Ralstonia spp.; the indirect inhibitory effect was dominant. Our results indicated that the co-application of humic acid and chitosan enhanced the interaction between soil bacterial communities and inhibited the growth of Ralstonia spp. by promoting beneficial microbial groups. This has practical application potential in the reasonable management of tomato facility fields and the control of soil-borne diseases. |
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