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| Isolation and molecular identification of nematodes intercepted from imported Netherlands′ lily bulbs |
| Received:November 10, 2022 Revised:December 06, 2022 |
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| KeyWord:Netherlands;lily bulb;nematode;molecular identification |
| Author Name | Affiliation | E-mail | | ZHANG Yinyin | Technology Center of Dalian Customs, Dalian 116001, China | | | HEI Duoer | Technology Center of Dalian Customs, Dalian 116001, China | | | LIU Yueting | Technology Center of Dalian Customs, Dalian 116001, China | | | LI Xin | Technology Center of Dalian Customs, Dalian 116001, China | 14826155@qq.com |
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| Abstract: |
| The increasing import of the Netherlands′ lily bulbs over the years has meant introduction of a variety of pests into the country; and one such nematode pest has turned into risk, endangering the national biosafety. Quarantine and identification of nematodes are thus imperative. This paper provides reference for the quarantine of imported seedlings and flowers to various ports and the supervision of planting sites. In this study, morphology（Beeman funnel method）, combined with molecular biological methods（DNA extraction and PCR amplification）, were used to quarantine and identify plant parasitic nematodes isolated from lily bulbs. The results showed that three species of plant parasitic nematodes were found：Pratylenchus penetrans, Aphelenchoides blastophthorus, and Aphelenchus avenae. The results also indicated that the quantity of nematodes isolated from the imported lily bulbs was limited, with most being larvae, as is it difficult to accurately identify nematodes based on their morphology, this process needed to be supplemented by molecular biological methods to ensure the accuracy of the results. |
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