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| Insecticidal activity and control effect of different biological preparations against Meloidogyne incognita |
| Received:November 10, 2022 Revised:December 13, 2022 |
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| KeyWord:tomato root-knot nematode disease;Meloidogyne incognita;biological control;Bacillus;wood vinegar |
| Author Name | Affiliation | E-mail | | LI Yunchao | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | LI Hongtao | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | lihongtao1314@yeah.net | | JI Hua | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | LI Junhua | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | LI Chaonan | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China
College of Plant Protection, Hebei Agricultural University, Baoding 071000, China | | | ZHANG Li | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | JIN Xiaoyan | Sino Green Agri-Biotech Co. Ltd., Beijing 102101, China | |
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| Abstract: |
| This study explores the insecticidal activity and control effect of different biological agents on Meloidogyne incognita. Ten Bacillus species and two kinds of Prunus armeniaca（apricot）shell wood vinegar were isolated and cultured in our laboratory and were tested for their insecticidal activity screening against Meloidogyne incognita. The control efficacy of the selected agents was verified using greenhouse pot-grown and field-grown tomatoes as testing plants. The indoor bioassay verification test showed that the fermentation broths of four Bacillus strains and the wild P. armeniaca shell wood vinegar had good poisoning effect on Meloidogyne incognita. The corrected mortalities for the Bacillus fermentation broths were 84.9% for strain XYG169, 100% for BMT295, 97.9% for strain BMT256, and 80.7% for the YLT74. The corrected mortality rates of nematodes with 100-fold, 200-fold, 400-fold, 600-fold and 800-fold diluted solutions of P. armeniaca shell wood vinegar were 100%, 100%, 92.8%, 65.4%, and 42.5%, respectively. The pot experiment in the greenhouse showed that the control effect of the 300-fold solution of P. armeniaca wood vinegar added with 4 g of BMT295 powder was the most effective, with a relative control effect of 74.00%. The field experiment showed that the same treatment of 300-fold solution had the best control effect on Meloidogyne incognita, with a relative control effect of 64.54%. The results of indoor bioassay and pot and field experiment revealed that the BMT295 powder mixed with P. armeniaca wood vinegar had a noticeable control effect, which could be used as a biological nematicide for further field experiment and popularization. |
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