|
| Isolation and identification of root rot pathogen of Atractylodes chinensis and screening of biocontrol bacteria |
| Received:November 08, 2022 Revised:November 29, 2022 |
| View Full Text View/Add Comment Download reader |
| KeyWord:Atractylodes chinensis;root rot;Fusarium oxysporum;biocontrol bacillus;medicinal plant |
| Author Name | Affiliation | E-mail | | LI Chaonan | College of Plant Protection, Hebei Agricultural University, Baoding 071000, China
Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | LI Hongtao | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | lihongtao1314@yeah.net | | LI Yunchao | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | LI Junhua | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | JI Hua | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | ZHANG Li | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | | | WANG Lin | Institute of Biotechnology and Food Science, Hebei Academy of Agriculture and Forestry Sciences, Shijiazhuang 050051, China | |
|
| Hits: 1640 |
| Download times: 1559 |
| Abstract: |
| To identify the pathogen of root rot of Atractylodes chinensis, pathogenic microorganisms were isolated from the tuber of Atractylodes chinensis in Chengde, and the pathogenicity of the pathogenic microorganisms was verified via back-grafting. The isolated pathogenic bacterium with pathogenicity was identified as Fusarium oxysporum. A total of 94 rhizosphere microorganisms were isolated from the rhizosphere soil of healthy medicinal plants, and two biocontrol strains, strain SFJ-27 and strain SFJ-41, were obtained through plate antagonism screening. Plate antagonism test results showed that the inhibition rates of the two biocontrol strains were above 50%. It was identified as Bacillus subtilis and Bacillus velezensis according to molecular biology and morphology. Pot experiments showed that strain SFJ-27 and strain SFJ-41 had significant biocontrol effects on root rot of Atractylodes chinensis, with control effects of 52.27% and 68.19%, respectively. Studies have shown that Fusarium oxysporum can cause root rot of Atractylodes chinensis and Bacillus subtilis and Bacillus velezensis have preventive effect on the root rot of Atractylodes chinensis. |
|
|
|
