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| Analysis of the dose-effect of biochar on the microbial community structure and environmental factors in tetracycline contaminated soils |
| Received:April 28, 2022 |
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| KeyWord:tetracycline;biochar;biodegradable;microbial community structure |
| Author Name | Affiliation | E-mail | | LI Huijun | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | WEI Ting | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | HUANG Fengcheng | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | CHEN Yijie | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | LI Gaoyang | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | ZHANG Weijian | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | WU Weijian | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | LIN Zhong | College of Chemistry and Environmental Sciences, Guangdong Ocean University, Zhanjiang 524088, China | | | ZHEN Zhen | College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang 524088, China | zz19830302@163.com |
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| Abstract: |
| In order to study the in situ remediation effect of different doses of biochar on tetracycline contaminated soils, this study used bagasse as raw material to prepare biochar. The removal efficiency of tetracycline, soil physicochemical properties, enzyme activities and degradation microorganisms during the remediation process were determined by adding different doses（1%, 2%, 3%）of biochar. The results showed that biochar application significantly accelerated the degradation of tetracycline in the soil, and tetracycline contaminated soil + 2% biochar （79.50%） was significantly higher than tetracycline contaminated soil + 3% biochar（62.50%） and tetracycline contaminated soil + 1% biochar（50.30%）. The application of biochar significantly increased the pH of the soil and contents of organic matter and humus. After incubation, compared with tetracycline contaminated soil, tetracycline contaminated soil + 1% biochar, tetracycline contaminated soil + 2% biochar, and tetracycline contaminated soil + 3% biochar resulted in significant increases in soil pH （0.46, 0.54, 0.80）, organic matter（1.37, 2.82, 5.12 g · kg-1）, and humus（4.48, 6.55, 5.21 g · kg-1）, respectively. In addition, biochar treatment significantly increased soil urease, sucrase, catalase, and dehydrogenase activities, with tetracycline contaminated soil + 2% biochar showing the best performance. In the biochar treatment, the abundance of tetracycline-potentially degrading bacteria, including Achromobacter, Sphingomonas, Stenotrophomonas, Trichosporon, Shewanella, Pseudomonas, and Klebsiella, were significantly increased, and tetracycline contaminated soil +2% biochar treatment had the best increasing effect on the abundance of potentially degrading bacteria. In conclusion, biochar enhances soil physiochemical properties, enzyme activity, and the abundance of potentially degrading bacteria and accelerates the biodegradation of tetracycline. As a carbon-rich material with low cost and good remediation effect, biochar has a great application potential in in situ remediation of tetracycline-contaminated sites. |
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