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| Changes in a multidrug-resistant bacterial community and its antibiotic resistance characteristics in pig farms after the ban on antibiotic growth promoters |
| Received:September 01, 2022 |
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| KeyWord:antibiotics|multi-drug resistant bacteria|livestock farm|animal feces |
| Author Name | Affiliation | E-mail | | HOU Jinjiang | College of Forestry, Northeast Forestry University, Harbin 150040, China
Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | SUN Xingbin | College of Forestry, Northeast Forestry University, Harbin 150040, China | sunxingbin1025@163.com | | GAO Haoze | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | WANG Xuming | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | GUO Yajie | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | GAO Min | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | | | QIU Tianlei | Beijing Agricultural Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China | qqray01@126.com |
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| Abstract: |
| Due to the frequent use of antibiotics, multidrug-resistant bacteria in farm manure may pose ecological risks to natural environments. To understand the changes in multidrug-resistant bacterial communities in pig farms after the ban on antibiotic growth promoters（AGPs）, we collected manure samples before and after the AGPs ban took effect, and counted, isolated, and identified the multidrug-resistant bacteria. We analyzed the community structure of multidrug-resistant bacteria by 16S rRNA gene amplicon sequencing. Multidrug-resistant strains present before and after the AGPs ban were analyzed by drug sensitivity testing. The residual concentration of AGPs（mainly oxytetracycline）significantly affected the distribution of multidrug-resistant bacteria, and after the AGPs ban the species abundance of multidrug-resistant bacteria in pig manure significantly decreased. The multidrug-resistant bacteria in pig manure mainly belonged to the four phyla Proteobacteria, Actinobacteria, Firmicutes, and Bacteroidetes. Escherichia was the dominant multidrug-resistant genus in all samples, and Kurthia, Providencia, Leucobacter, Corynebacterium, Ignatzschineria, Lysinibacillus, Staphylococcus, and other multidrug-resistant genera decreased in relative abundance after the AGPs ban. The AGPs ban reduced rates of resistance to feed antibiotics including tetracyclines, chloramphenicol, and macrolides in multidrug-resistant bacteria in pig manure. However, it also increased their rate of resistance to therapeutic antibiotics including aminoglycosides, cephalosporins, and rifampicin. |
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