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| Mechanism of foliar spraying of 2, 3-dimercaptosuccinic acid(DMSA) affects cadmium content in shoots of rice seedlings |
| Received:August 30, 2022 |
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| KeyWord:rice;cadmium;2, 3-dimercaptosuccinic acid(DMSA);shoot base;cell wall |
| Author Name | Affiliation | E-mail | | WANG Xiaoli | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WANG Changrong | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Zhongqi | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | HUANG Yizong | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | KONG Weiyong | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | HUANG Yongchun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | nylab@126.com | | LIU Bin | Agricultural Resource and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 537000, China | gxliubin@126.com |
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| Abstract: |
| In order to explore the potential mechanism of foliar spraying of 2, 3-dimercaptosuccinic acid(DMSA) to reduce the cadmium (Cd) content in the stems and leaves of rice seedlings, the accumulation of Cd in different organs of the seedlings and the effects on Cd stress were studied in an artificial climate chamber by using a hydroponic experiment. The results showed that with the increase of DMSA spraying concentration, the Cd content of the shoot base significantly increased. When the DMSA spraying concentration reached 1.0 mmol· L-1, the Cd content of the shoot base significantly increased by 57.3% compared with the control treatment, and the transfer factor of Cd from the shoot base to the shoot decreased by 52.7%. It was found that when the concentration of DMSA reached 1.0 mmol·L-1, insoluble Cd content increased significantly by 80.8% compared with the control treatment. The subcellular distribution test showed that the Cd content of the cell wall increased significantly with the DMSA spraying concentration. When the spraying concentration reached 1.0 mmol· L-1, the Cd content of the cell wall was about 2.1 times that of the control group. Further analysis of the Cd content of cell wall components showed that the Cd content in pectin increased with DMSA spraying concentration, and the highest increase was 99.5% when compared with the control treatment. In addition, after spraying DMSA, total phytochelatin and glutathione content of the stem base showed significant increasing trends, and the highest measured values were 2.2 times and 3.1 times those of the control group, respectively. After spraying DMSA, the activities of SOD and CAT in shoots increased to 3.0 times and 2.7 times, respectively. A fluorescence labeling test showed that the content of hydrogen peroxide in leaves also decreased significantly. The above results showed that the spraying of DMSA significantly increased the content of sulfhydryl compounds in the stem base of rice seedlings, improved the ability to intercept cadmium, reduced the efficiency of Cd transport from the stem base to the shoots of rice seedlings, and significantly decreased Cd content in the shoots of rice seedlings. Furthermore, this study shows that the spraying of DMSA can significantly alleviate Cd stress in rice seedlings. |
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