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| Effects of ozone pollution and EDU spraying on the phyllospheric bacterial community of wheat plants |
| Received:August 04, 2022 |
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| KeyWord:ozone;ethylenediurea;wheat;phyllospheric bacteria;community structure;biodiversity |
| Author Name | Affiliation | E-mail | | SU Yi | School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China | | | CHENG Cheng | School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China | | | WANG Qi | School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China | qiwang@nuist.edu.cn | | LIU Yuanyuan | School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China | | | XU Yansen | School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China | | | FENG Zhaozhong | School of Applied Meteorology, Nanjing University of Information Science & Technology, Nanjing 210044, China | zhaozhong.feng@nuist.edu.cn |
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| Abstract: |
| In order to study the effects of elevated O3, ethylenediurea(EDU), and their interactions with the phyllospheric microbial community, Triticum aestivum L. Nongmai88 was grown in the China O3 Free-Air Concentration Enrichment(O3-FACE) platform under either ambient atmospheric O3 concentration(A) or 1.5 times ambient atmospheric O3(E), and the foliage was sprayed with 450 mg·L-1 EDU or water every ten days from March 1st to May 13th. Compared with A treatment, plant height decreased by 9.4% under E treatment, while plant height increased by 11.0% when EDU was sprayed on leaves under E treatment. Based on alpha diversity analysis of the phyllospheric bacterial community, elevated O3, EDU, and their interactions had no significant effect on alpha diversity indices. Both NMDA and PCoA results indicated that the structure of the phyllospheric bacterial community was significantly affected by elevated O 3 and EDU, respectively. At the phylum level, the dominant phyllospheric bacteria included Proteobacteria(84.7%-94.8%) (with Gammaproteobacteria accounting for 75.2%-94.3%), Bacteroidetes(1.3%-13.1%), and Firmicutes(1.6%-5.4%). Under E treatment, EDU significantly reduced the relative abundance of Gammaproteobacteria and increased the relative abundance of Bacteroidetes compared with the control group(water treatment). In addition, the relative abundance of the abundant genera also changed significantly. Compared with A treatment, E significantly decreased the relative abundance of Pseudomonas and Serratia by 50.6%-57.2% and 41.4%-65.5%, respectively. Under E treatment, EDU reduced the relative abundance of Erwinia by 73.0% compared with the control group. In conclusion, both elevated O3 and EDU change the phyllospheric bacterial community structure of wheat plants, although they have no effect on alpha-diversity. EDU may improve the tolerance of wheat to O3 stress by increasing the abundance of environmentally adaptable microbiota. |
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