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| Effects of foliar sprayed S-allyl-L-cysteine on arsenic transport in rice plants |
| Received:January 27, 2023 |
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| KeyWord:rice;arsenic;S-allyl-L-cysteine;transporter;gene expression |
| Author Name | Affiliation | E-mail | | LANG Yaozhen | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China
School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China | | | LIU Bin | Agricultural Resource and Environment Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China | | | WANG Changrong | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Zhongqi | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | KONG Weiyong | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Yuemin | School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China | lyuemn@126.com | | HUANG Yongchun | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | nylab@126.com |
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| Abstract: |
| Dietary rice is the main source of inorganic arsenic（iAs）with a high carcinogenic risk for the population, therefore developing countermeasures to reduce the arsenic（As）content in rice is of great importance. Here, we investigated the effect of foliar spraying of Sallyl-L-cysteine（SAC）on the total As content in rice grains and nutritive organs during flowering and identified the potential molecular mechanism of SAC using the main rice cultivar "ZhongZao 35" in southern China. The experimental results showed that the As content in grains and roots significantly decreased by 42.3% and 20.6%, respectively, when the SAC spraying concentration reached 0.2 mmol·L-1, but significantly increased in flag leaves by 72.4%. Fluorescence staining showed that the H2O2 content in flag reduced significantly, while the SOD and CAT enzyme activities significantly increased by 61.8% and 105.3%, respectively. The genes encoding Lsi6 and Lsi3 transporters in the first rice node were significantly downregulated by 59.8% and 36.3%, respectively, after SAC spraying; thus, significantly reducing the ability of rice to unload AsⅢ from the vascular stream leading to the expansion of vascular bundle in the flag leaves and load AsⅢ into the diffused vascular bundle connected to the rice spike. Moreover, it resulted in a significant decrease in the total As content in the grains and a significant increase in the flag leaves. The genes encoding OsPCS1, a phytochelatins-（PCs）-synthesizing enzyme, and OsABCC1, an AsⅢ transporter in cell vacuole, were significantly upregulated by 57.6% and 61.0% respectively, indicating that SAC spraying increased the ability of flag leaves to synthesize PCs and compartmentalize AsⅢ into the vacuole, thereby reducing AsⅢ stress in the leaves. The genes encoding Lsi1, Lsi2, and Lsi3 transporters in rice roots were significantly downregulated by 27.2%, 23.8%, and 29.5%, respectively, indicating that the uptake and transport capacity of AsⅢ in rice roots was reduced, which further minimized the loading capacity of AsⅢ into the xylem ducts. In conclusion, spraying SAC reduces As content in grains and roots by regulating the expression of genes encoding AsⅢ related transporter and alleviates As stress. |
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