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| In-situ purification of aquaculture water using an immobilized bacterial agent and its influence on the microbial community structure |
| Received:November 04, 2022 |
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| KeyWord:immobilized bacterial agent;aquaculture water;in-situ remediation;community structure |
| Author Name | Affiliation | E-mail | | CAI Xuyi | College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China | | | TIAN Yaxiong | College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China | | | CHEN Panyi | College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China | | | GE Pengbiao | Suzhou Dingxingsiwo Aquaculture Equipment Co., Ltd., Suzhou 210095, China | | | ZHANG Liuliu | Jiangsu NAK Bioengineering Co., Ltd., Huai' an 211700, China | | | LI Juanying | College of Marine Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China
Shanghai Engineering Research Center of River and Lake Biochain Construction and Resource Utilization, Shanghai 201702, China | jyli@shou.edu.cn |
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| Abstract: |
| In order to understand the effect of the in-situ application of an immobilized bacterial agent on the purification of aquaculture water and the microbial community structure in the aquaculture environment, the concentration changes of pollutants（including CODMn, ammonia, and TP） in aquaculture wastewater were analyzed during in-situ remediation in the present study. Additionally, the characteristics of the microbial community in the aquaculture environment were evaluated using a high-throughput sequencing method. The results showed that the removal of CODMn and ammonia in the experimental aquaculture pond was significantly improved（P<0.05）. The removal rate of CODMn increased from 26.14% to 52.49%, and the removal rate of ammonia nitrogen increased from 76.21% to 90.73%. The concentration of CODMn was lower than the discharge limit of aquaculture wastewater（15 mg·L-1）, ammonia, and TP at a low level（< 0.5 mg·L-1）. The Shannon index and Chao index of microbial diversity in the experimental group were significantly higher than those of the control group（P<0.05）. The addition of an immobilized bacterial agent increased the number of the total OTU and the unique OTU in the experimental group. The number of common OTUs between the sediment and water from the experimental group was more than those in control group. Principal coordinate analysis（PCoA）showed that the dominant microbial population in the experimental group changed significantly compared with the control group. The increase of Proteobacteria promoted the transformation of ammonia, and Actinobacteria, being responsible for the decay of animal and plant remains, was reduced, which might be related to the greatly decreased COD Mn in the experimental group. Meanwhile, the reduction of Bacteriidota was also caused by improved water quality in the experimental group. Changes in relative abundance at the genus level also reflected that the in-situ application of an immobilized bacterial agent optimized the environmental microbial community structure. |
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