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| Quantification of nitrogen reduction of each component in constructed wetland and functional gene analysis |
| Received:August 01, 2023 |
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| KeyWord:constructed wetland;nitrogen isotope;microbial community;nitrogen metabolism;functional gene |
| Author Name | Affiliation | E-mail | | GAO Jingtian | School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Autonomous Region Collaborative Innovation Center for Ecological Protection and Comprehensive Utilization of Inner Mongolia Section of Yellow River Basin, Baotou 014010, China | | | TANG Shike | School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Autonomous Region Collaborative Innovation Center for Ecological Protection and Comprehensive Utilization of Inner Mongolia Section of Yellow River Basin, Baotou 014010, China | | | LIU Zheming | School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Autonomous Region Collaborative Innovation Center for Ecological Protection and Comprehensive Utilization of Inner Mongolia Section of Yellow River Basin, Baotou 014010, China | | | ZHANG Peng | School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Autonomous Region Collaborative Innovation Center for Ecological Protection and Comprehensive Utilization of Inner Mongolia Section of Yellow River Basin, Baotou 014010, China | | | YU Fengping | School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Autonomous Region Collaborative Innovation Center for Ecological Protection and Comprehensive Utilization of Inner Mongolia Section of Yellow River Basin, Baotou 014010, China | | | LI Weiping | School of Energy and Environment, Inner Mongolia University of Science and Technology, Baotou 014010, China
Autonomous Region Collaborative Innovation Center for Ecological Protection and Comprehensive Utilization of Inner Mongolia Section of Yellow River Basin, Baotou 014010, China | sjlwp@163.com |
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| Abstract: |
| Six groups(three groups without plants) of constructed wetland systems with substrates of ceramic pellets+cinder, ceramic pellets+zeolite, and zeolite+cinder were constructed to investigate the effect of nitrogen removal in Baotou Nanhai Lake and explore the contribution of nitrogen reduction of each component in a constructed wetland and the mechanism of microbial nitrogen removal. Nitrogen stable isotope tracing technology was used to quantify the contribution of plants, substrates, and microorganisms to nitrogen removal in a constructed wetland. High-throughput sequencing technology was used to investigate the microbial community structure and nitrogen metabolism functional genes in the system. The results showed that the constructed wetland with plants comprising ceramic+cinder was the most effective in nitrogen removal, with removal rates of (41.18±2.61)%, (50.44±2.63)%, (40.93±2.32)%, and (74.34±1.97)% for TN, NH4+-N, NO3--N, and NO2--N, respectively. 15N tracing revealed that the contribution rates of plants, substrates, and microorganisms to the NO3--N removal in the constructed wetland system were 27.74%, 48.43%, and 23.83%, respectively. High-throughput sequencing and PICRUSt2 functional prediction results revealed that plants significantly impacted microbial composition and metabolic pathways. The dominant phylum was Proteobacteria, most of whose genera are involved in the nitrogen cycle and are crucial in biological denitrification. The abundance of nirS on the substrate and nirK in the plant roots of a planted constructed wetland system increased significantly. Plants effectively promoted the denitrification of microorganisms in constructed wetland systems. |
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