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| Effects of long-term different fertilization on the structure and diversity of CO2-assimilating bacterial communities in wheat field soils |
| Received:November 03, 2023 |
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| KeyWord:fertilization;wheat field;carbon-fixing bacteria;cbbL gene;community structure;high-throughput sequencing |
| Author Name | Affiliation | E-mail | | HAI Xiang | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Qianlong | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | PANG Bo | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | WANG Hui | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | YANG Dianlin | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | | | LIU Hongmei | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | liuhongmei@caas.cn | | ZHAO Jianning | Agro-Environmental Protection Institute, Ministry of Agriculture and Rural Affairs, Tianjin 300191, China | zhaojn2008@163.com |
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| Abstract: |
| The study aimed to investigate the effects of different fertilization methods on the soil CO2-assimilating bacterial community structure and diversity in wheat fields in the North China Plain, providing data support for rational fertilization in agricultural fields in North China. Using the long-term fertilization experimental of the Environmental Monitoring Institute of the Ministry of Agriculture and Rural Affairs as the platform, high-throughput sequencing technology was employed to study the effects of five different fertilization methods：control without fertilization（CK）, application of organic fertilizer alone（M）, application of nitrogen fertilizer alone（N）, application of inorganic fertilizer alone（NPK）, and combined application of organic and inorganic fertilizers（MNPK）, on the soil CO2-assimilating bacterial community structure and diversity in wheat fields. The results showed that：The Shannon diversity index of soil CO2-assimilating bacteria was highest under the MNPK treatment, significantly higher than those under M, N, NPK, and CK treatments; there were no significant differences in the Shannon indices between M, N, and NPK treatments and CK. The main factors influencing the α-diversity index of CO2-assimilating bacteria were microbial biomass carbon, microbial biomass nitrogen, nitrate nitrogen, ammonium nitrogen, total nitrogen, and pH value. The relative abundance of dominant bacterial groups in the CO2-assimilating bacteria community of farmland soils in the North China Plain had changed. At the phylum level, Proteobacteria（96.11%-99.97%）was the dominant group; compared with CK, the four fertilization treatments significantly decreased the relative abundance of the Proteobacteria. At the class level, the dominant classes were Gammaproteobacteria（68.31%-89.14%）, Alphaproteobacteria（4.29%-27.32%）, and Betaproteobacteria （1.62%-7.71%）; compared with CK, the N, NPK, and MNPK treatments significantly reduced the relative abundance of Gammaproteobacteria, while the M treatment significantly increased it; the NPK and MNPK treatments significantly increased the relative abundance of Alphaproteobacteria, while the M treatment significantly decreased it; all four fertilization treatments significantly increased the relative abundance of Betaproteobacteria.（3）Redundancy analysis results indicated that soil pH, microbial biomass carbon, microbial biomass nitrogen, nitrate nitrogen, ammonium nitrogen, total soil organic carbon, and C/N ratio were the main factors affecting the changes in the community characteristics of soil CO2-assimilating bacteria. These results suggest that long-term combined application of organic and inorganic fertilizers is more conducive to microbial carbon sequestration in soil; a decrease in soil pH and nutrient accumulation are important reasons for changes in the soil CO2-assimilating bacteria community and diversity. |
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