Advanced Search
Analyzing Soil Microbial Community Structure Diversity from Jianhu Wetland Lakeside Zone Using PCR-DGGE Technique
  
View Full Text  View/Add Comment  Download reader
KeyWord:PCR-DGGE; Jianhu wetland; rhizosphere microorganisms; diversity
Hits: 3290
Download times: 2960
Abstract:
      This study analyzed the bacterial community structure from the rhizosphere of four plants found in the Jianhu wetland lakeside zone using PCR-DGGE technique. The results showed that soil samples collected from plant rhizosphere and non-rhizosphere displayed significant differences in the Shannon-wiener index,richness and evenness, respectively. With the highest bacterial diversity was found in Zizania latifolia rhizosphere soil, plant community did influence microbial diversity. Community structure similarity was higher in plant rhizosphere than non-rhizosphere soil. Based on 82% community similar levels, two major groups were categorized, with rhizosphere bacteria and Zizania latifolia non-rhizosphere bacteria as one group, and the rest as the other group. Plant community did influence microbial community structure. Additionally, according to the results of sequence analysis of DGGE dominant bands, all bacterial strains were phylogenetically closed to some uncultured microbes, including Proteobacteria, Oxalobacteraceae, Janthinobacterium, Duganella, Escherichia, and Streptococcus. Plant rhizosphere soil nitrogen and phosphorus contents were different. The highest nitrogen and phosphorus contents were found in Zizania latifolia rhizosphere soil. Soil bacterial diversity positively related to soil organic matter, total nitrogen and total phosphorus contents, but negatively related to soil pH.