|
| 哈密瓜连作根际土壤中氮、磷循环功能基因的赋存特征 |
| Occurrence characteristics of rhizosphere nitrogen and phosphorus cycling functional genes in continuous cropping of Hami melon |
| 投稿时间：2025-03-14 |
| DOI：10.13254/j.jare.2025.0210 |
| 中文关键词: 氮循环基因，磷循环基因，连作，哈密瓜，宏基因组学，共现网络 |
| 英文关键词: nitrogen cycling gene, phosphorus cycling gene, continuous cropping, Hami melon, metagenomics, co-occurrence network |
| 基金项目:新疆维吾尔自治区重大科技专项（2022A02007-4）；新疆农业大学研究生科研创新项目（XJAUGRI2024033） |
|
| 摘要点击次数: 283 |
| 全文下载次数: 42 |
| 中文摘要: |
| 持续单一类型的作物种植会加剧土壤生态环境的恶化，进而影响土壤中养分平衡和作物生长，然而目前并无关于连作对哈密瓜根际土壤养分循环功能基因影响的相关研究。本研究以新疆特产哈密瓜为研究对象，通过宏基因组分析了不同连作年限（8、15年和25年）哈密瓜根际土壤中氮、磷循环功能基因的分布特征及其与土壤化学性质的相关性。结果表明：随着哈密瓜种植年限的增加，土壤pH显著下降；与此同时，电导率、碱解氮、速效磷、速效钾、全磷和全钾等化学因子显著增加，表明长期连作导致土壤盐分和养分积累。PCoA分析结果表明不同连作程度哈密瓜根际土壤氮、磷循环功能基因赋存特征显著不同。短期连作（如8年）有利于氮同化途径的相关基因表达，而长期连作（如25年）则更有利于氮吸收和磷缺乏响应调控的相关基因表达。通过共现网络拓扑结构特征分析，鉴定出1个氮循环关键功能基因以及6个磷循环关键功能基因，且连作显著增加了有机磷矿化过程相关的关键功能基因（phy、phnW及phnI）。相关性分析结果表明，氮循环关键基因nasA与土壤pH呈显著正相关；磷循环关键基因phnW与土壤电导率和速效磷含量呈显著正相关；而pit和pstB基因与部分营养指标和连作年限呈负相关。本研究揭示了长期连作对哈密瓜根际土壤氮、磷循环功能基因的影响机制，短期连作可通过优化氮肥管理来维持氮同化效率，而长期连作则需优化磷肥管理以缓解磷的吸收与转运。这些发现为优化哈密瓜种植模式、改善土壤质量提供了基础数据。 |
| 英文摘要: |
| Continuous monoculture of a single crop type can exacerbate the deterioration of soil ecological environments, thereby affecting the balance of soil nutrients and crop growth. However, there is currently no research on the impact of continuous cropping on the nitrogen and phosphorus cycling functional genes in the rhizosphere soil of Hami melon. This study focused on Hami melon, a specialty of Xinjiang, as the research object and analyzed the distribution characteristics of nitrogen and phosphorus cycling functional genes in the rhizosphere soil of Hami melon with different continuous cropping years（8, 15, and 25 years）and their correlation with soil chemical properties using metagenomic analysis. The results showed that with the increase in the cropping years of Hami melon, the soil pH significantly decreased, while the electrical conductivity, available nitrogen, available phosphorus, available potassium, total phosphorus, and total potassium significantly increased, indicating that long-term continuous cropping led to the accumulation of soil salinity and nutrients. PCoA results showed that the characteristics of nitrogen and phosphorus cycling functional genes in the rhizosphere soil of Hami melon with different continuous cropping levels were significantly different. Short-term continuous Hami melon cropping（e.g., 8 years）was more conducive to the expression of genes related to nitrogen assimilation pathways, while long-term continuous cropping（e.g., 25 years）was more conducive to the expression of genes related to nitrogen uptake and phosphorus deficiency response pathways. Through co-occurrence network topology analysis, one key functional gene involved in nitrogen cycling and six key functional genes involved in phosphorus cycling were identified. Continuous Hami melon cropping significantly increased the key functional genes related to organic phosphorus mineralization processes（phy, phnW, and phnI）. Correlation analysis results showed that the key nitrogen cycling gene nasA was significantly positively correlated with soil pH. The key phosphorus cycling gene phnW was significantly positively correlated with soil EC and available phosphorus content, while the pit and pstB genes were negatively correlated with some nutritional indicators and continuous cropping year. This study reveals the impact mechanisms of long-term continuous cropping on the nitrogen and phosphorus cycling functional genes in melon rhizosphere soil of Hami melon. Short-term continuous cropping can maintain nitrogen assimilation efficiency by optimizing nitrogen fertilizer management, while long-term continuous cropping requires optimizing phosphorus fertilizer management to alleviate phosphorus uptake and transport. These findings provide basic data for optimizing Hami melon planting patterns and improving soil quality. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
