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| 不同钾和硼水平对棉花叶片逆境生理及其细胞壁硼的影响 |
| Effect of Different Levels of Potassium and Boron on Stress Physiology and Cell Wall Boron Content of Cotton Leaf |
| Received:June 20, 2015 |
| DOI：10.13254/j.jare.2015.0154 |
| 中文关键词: 棉花;钾;硼;逆境生理;细胞壁硼 |
| 英文关键词: cotton;potassium;boron;stress physiology;boron in the cell wall |
| 基金项目:国家自然科学基金(40801112);公益性行业(农业)科研专项(201203013) |
| Author Name | Affiliation | E-mail | | WU Xiu-wen | Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China
Key Laboratory of Arable Land Conservation, Ministry of Agriculture, Wuhan 430070, China | | | HAO Yan-shu | Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China
Key Laboratory of Arable Land Conservation, Ministry of Agriculture, Wuhan 430070, China | | | LEI Jing | Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China
Key Laboratory of Arable Land Conservation, Ministry of Agriculture, Wuhan 430070, China | | | JIANG Cun-cang | Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China
Key Laboratory of Arable Land Conservation, Ministry of Agriculture, Wuhan 430070, China | jcc2000@mail.hzau.edu.cn |
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| 中文摘要: |
| 为了揭示钾、硼对棉花叶片细胞膜及硼利用的影响,本试验以棉花作为研究材料,采用营养液培养方式,研究了不同钾、硼处理叶片逆境下细胞膜及与细胞壁结合的硼比例的变化。结果表明,施钾水平为20 mg·L-1时,缺硼(0 mgB·L-1)阻碍棉花地上部生长和干物质的积累,且与正常硼(0.2 mgB·L-1)相比,叶片逆境生理指标均显著升高,束缚态硼相对含量、R值(半束缚态硼/自由态硼)、细胞壁提取率和叶片总细胞壁硼占叶片硼的比例均有所升高;施钾水平为2 mg·L-1时,缺硼叶片逆境生理指标、自由态硼和半束缚态相对含量及R值较正常硼处理也均显著升高,但束缚态硼相对含量和叶片总细胞壁硼占叶片硼的比例降低。因此,适钾与低钾时,缺硼均会明显破坏细胞膜透性。适钾条件下,缺硼导致细胞壁增厚,在细胞壁与果胶多糖结合的硼的比例升高;而低钾时,缺硼对于叶片细胞壁提取率无显著影响,进入叶片细胞质的硼的比例升高,但与果胶多糖结合的硼的比例降低。 |
| 英文摘要: |
| To find out the effect of potassium(K) and boron(B) on cotton leaf cell membrane and B distribution and utilization, the membrane relative permeability, MDA, Pro, the content of free B, semi-bound B and bound B and the content of B in cell wall of cotton leaf were analyzed under different K levels with solution culture method in this study. The results showed that in normal K(20 mgK·L-1), B deficiency(0 mgB·L-1) hindered the normal growth and dry mass of shoots, in addition, the membrane relative permeability, the content of MDA and Pro significantly increased compared with the normal B(0.2 mgB·L-1), and the relative concentration of bound B, R value, cell wall material and the ratio of total cell wall B/leaf B increased by 10.32%, 21.28%, 31.35% and 31.35%, respectively. In contrast, under low K(2 mgK·L-1) supply, B deficiency produced a very significant decrease in the relative concentration of bound B and the ratio of total cell wall B/leaf B. The above results showed that under either K-deficient or K-sufficient condition, B deficiency damaged the cotton leaf cell membrane and cell membrane permeability. In normal B-supplied plants, lacking of B induced more B into the cytoplasm, but increased the proportion of B that combined with the pectic polysaccharides in cell wall. However, under K-deficient treatment, the proportion of B cross-linked pectic polysaccharides in cell wall decreased. |
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