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| 滇西患病乌头根际与根系细菌和真菌多样性分析 |
| Bacterial and fungal diversity analysis in the rhizosphere and root of diseased aconite（Aconitum napellus Debx.）in western Yunnan |
| Received:January 03, 2025 |
| DOI：10.13254/j.jare.2025.0007 |
| 中文关键词: 乌头，根腐病，微生物组成，微生物多样性，土壤理化性质，高通量测序 |
| 英文关键词: Aconitum napellus, root rot, microbial composition, microbial diversity, soil property, high-throughput sequencing |
| 基金项目:云南省重大科技专项计划项目（202302AA310027）；云南省特经作物绿色发展中越国际联合实验室项目（202403AP140013）；云南省农业基础研究联合专项（202401BD070001-052）；云南农业大学科研启动基金项目（S9012023030） |
| Author Name | Affiliation | E-mail | | Li Weichun | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | Dai Xingxun | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | Liu Lufeng | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | Zi Shuhui | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | Bao Li | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | Li Xuedong | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | | | Li Wanting | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | liwantingy@163.com | | Di Yining | College of Resources and Environment, Yunnan Agricultural University, Kunming 650201, China | 506903772@qq.com |
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| 中文摘要: |
| 本研究以滇西片区健康和患病乌头为研究对象，采用高通量测序技术对其根际与根系细菌和真菌群落结构组成差异及与环境因素之间的关系进行分析。结果表明：细菌和真菌群落组成与多样性在根际土、须根、表皮和块根4个部位存在显著差异，乌头患病后表皮和块根部位细菌多样性降低，须根和块根部位真菌多样性升高；须根和块根部位芽孢杆菌属（Bacillus）细菌和陶氏菌属（Tausonia）真菌的相对丰度显著降低，欧文氏菌属（Erwinia）细菌和镰孢菌属（Fusarium）真菌的相对丰度显著增加。冗余分析结果表明，土壤pH、全氮、全钾和硝态氮是影响乌头根际细菌和真菌群落组成的主要因素。属水平丰度排名前30的热图分析显示，芽孢杆菌属与土壤全钾呈显著正相关，而镰孢菌属与土壤速效磷和速效钾呈显著负相关。综上所述，欧文氏菌和镰孢菌在乌头须根和块根部位的富集是根腐病的关键致病因子，合理提升土壤速效磷、速效钾含量有助于芽孢杆菌等有益菌的富集，可为乌头根腐病防治提供科学依据。 |
| 英文摘要: |
| In this study, the high-throughput sequencing technology was employed to investigate the bacterial and fungal community structures and their associations with soil environmental factors in healthy and diseased aconite（Aconitum napellus Debx.）. The results showed shat，there were significant variations in the community composition and diversity across different compartments, including rhizosphere soil, fibrous roots, epidermis, and root tubers. The diversity of bacterial communities in the epidermis and root tubers in a diseased aconite exhibited a decline, whereas the diversity of fungal communities in fibrous roots and root tubers showed an increase. The fibrous roots and root tubers of diseased plants showed a marked reduction in the relative abundance of Bacillus and Tausonia, alongside a significant increase in the relative abundance of Erwinia and Fusarium. Redundancy analysis indicated that pH, total nitrogen，total potassium and nitrate nitrogen were the primary factors influencing the bacterial and fungal diversity in rhizosphere soil. The correlation heatmap analysis revealed that the abundance of Bacillus positively correlated with soil total potassium, while Fusarium abundance negatively correlated with available phosphorus, and nitrate nitrogen. In conclusion, the accumulation of Erwinia and Fusarium in the root system appears to be a primary factor contributing to the incidence of root rot. Enhancing the levels of available phosphorus, available potassium, and nitrate nitrogen in the soil is beneficial for the proliferation of beneficial bacteria, such as Bacillus. It provides a scientific basis for the prevention and management of A. napellus. root rot. |
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