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| 生态位还是基因型主导豌豆植株-土壤连续体中真菌群落组装机制？ |
| Niche or genotype dominates the assembly mechanisms of fungal communities in the pea-soil continuum? |
| Received:July 24, 2025 |
| DOI：10.13254/j.jare.2025.0754 |
| 中文关键词: β多样性分解，中性模型，溯源分析，物种替换，丰富度差异 |
| 英文关键词: β-diversity partitioning, neutral community model, source tracking analysis, species turnover, richness difference |
| 基金项目:宁波科技特派员团队项目（2023S213）；国家食用豆产业技术体系生态与土壤管理岗项目（CARS-08-G-09） |
| Author Name | Affiliation | E-mail | | Zhang Liquan | Cixi City Agricultural Technology Extension Center, Cixi 315300, China | | | Liu Qiong | State Key Laboratory for Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
International Science and Technology Cooperation Base for the Regulation of Soil Biological Functions and One Health of Zhejiang Province, Ningbo University, Ningbo 315211, China | | | Wang Yu | State Key Laboratory for Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
International Science and Technology Cooperation Base for the Regulation of Soil Biological Functions and One Health of Zhejiang Province, Ningbo University, Ningbo 315211, China | | | Xu Linying | Cixi City Agricultural Technology Extension Center, Cixi 315300, China | 283616641@qq.com | | Ge Tida | State Key Laboratory for Quality and Safety of Agro-Products, Ningbo University, Ningbo 315211, China
International Science and Technology Cooperation Base for the Regulation of Soil Biological Functions and One Health of Zhejiang Province, Ningbo University, Ningbo 315211, China | |
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| 中文摘要: |
| 为了明确宿主基因型与生态位在豌豆-土壤连续体真菌群落构建中的作用机制，本研究基于4个豌豆基因型和8个生态位（非根际土壤、根际土壤、根鞘土壤、根内、茎内、叶内、叶表和籽粒），采用β多样性分解量化物种替换与丰富度差异的贡献、中性群落模型评估随机/确定性过程的相对作用和溯源分析解析微生物迁移路径的方法，系统解析真菌群落结构、组装机制及潜在迁移路径。结果表明，生态位对真菌群落多样性具有显著影响，其中根鞘土壤具有最高的α多样性，而茎内和根内较低。群落结构差异主要由生态位驱动（R2=78.3%），远高于基因型（R2=0.8%）。β多样性分解结果表明，生态位间群落差异主要由物种替换引起（62.5%）。中性模型分析揭示，豌豆真菌群落整体以确定性过程主导，但地上部内生生态位（叶内、茎内和籽粒）拟合度较高（R2>0.7），表明其更受随机过程影响。溯源分析显示，真菌群落主要沿非根际土壤→根际土壤→根鞘土壤→根内→茎/叶内→籽粒的路径逐级向内部迁移，植物体形成显著的迁移筛选屏障：根鞘土壤向根内的迁移率仅为1.7%，而地上部内生生态位间的迁移率则超过89%。本研究系统揭示了生态位主导的群落分异格局与组装机制，为豆科作物微生物组的生态适应研究及其精准调控提供了理论依据。 |
| 英文摘要: |
| This study aimed to investigate the effects of host genotype and ecological niche on fungal community assembly along the pea-soil continuum. Fungal communities were analyzed from four pea genotypes across eight niches（bulk soil, rhizosphere soil, rhizosheath soil, root endosphere, stem endosphere, leaf endosphere, phylloplane, and seed）. Community dissimilarity, assembly mechanisms, and potential migration pathways were systematically investigated：β - diversity partitioning was used to quantify the contributions of species turnover versus richness differences; neutral community models were applied to evaluate the relative influence of stochastic and deterministic processes, and source-tracking analysis was performed to identify potential microbial migration routes. Fungal diversity and community structure were strongly influenced by niches, with the phylloplane showing the highest α-diversity, while root and stem endospheres had the lowest. Niche was the main factor driving community variation（R2=78.3%）compared with genotype（R2=0.8%）. Species replacement accounted for 62.5% of community differences among niches. Neutral model fitting revealed that overall fungal community assembly was predominantly deterministic; however, the stochastic processes played a larger role in aboveground endosphere niches（R2>0.7）. Source tracking suggested a potential migration of fungi from bulk soil to rhizosphere soil, then to rhizosheath soil, root endosphere, stem/leaf endosphere, and finally to seed. Notably, the migration rate from rhizosheath soil to root endosphere was only 1.7%, whereas it exceeded 89% among aboveground endosphere compartments. These findings suggest the strong selective filtration exerted by the pea host on fungal entry and dispersal. This study highlights the niche-dominated assembly processes and stepwise microbial migration within the pea -soil continuum, providing a foundation for understanding ecological adaptation and guiding the targeted management of legume-associated mycobiomes. |
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