| .典型岩溶生态系统土壤酶活性、微生物数量、有机碳含量及其相关性——以丫吉岩溶试验场为例[J].农业环境科学学报,2013,32(2):. |
| 典型岩溶生态系统土壤酶活性、微生物数量、有机碳含量及其相关性——以丫吉岩溶试验场为例 |
| Relationship Among Soil Organic Carbon, Enzyme Activities and Microbial Numbers in Typical Karst Ecosystem: A Case Study of Yaji Karst Experimental Site, China |
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| DOI：10.11654/jaes.2013.02.014 |
| 中文关键词: 峰丛洼地 土壤有机碳 酶活性 微生物数量 |
| 英文关键词: karst depression soil organic carbon enzyme activity microbial number |
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| 中文摘要: |
| 研究岩溶区土壤有机碳与微生物区系数量和土壤酶活性之间的关系有助于认识有机碳的积累机制。以丫吉岩溶试验场这一典型岩溶生态系统的洼地、坡地与垭口三种岩溶形态为研究样点，分别采集0~10、10~20 cm与20~30 cm土壤样本，采用浓硫酸-重铬酸钾外加热法测定土壤有机碳质量分数，用平板菌落计数法获取微生物区系数量，采用比色法分析土壤酶活性。结果表明，随着土壤剖面深度加深，土壤有机碳质量分数显著下降，在0~10 cm，洼地的土壤有机碳质量分数为40.10 g·kg-1，显著高于垭口的38.28 g·kg-1和坡地的24.42 g·kg-1；洼地土壤剖面从上至下细菌数量分别为3.26×106、2.73×106 CFU·g-1和1.15×106 CFU·g-1，均显著高于垭口和坡地；洼地土壤0~10 cm的脲酶活性为1.92 mg·（g·24 h）-1，显著高于垭口和坡地的1.12 mg·（g·24 h）-1和0.77 mg·（g·24 h）-1；碱性磷酸酶活性在洼地土壤剖面均显著高于垭口和洼地剖面。统计分析显示，不同类型的微生物数量和土壤酶活性与有机碳之间的关系在剖面上的表现并不一致，细菌数量、脲酶活性、归一化的微生物数量和归一化的土壤酶活性与土壤有机碳质量分数均呈显著正相关。以上研究结果表明，岩溶生态系统土壤有机碳积累下，土壤细菌数量、总微生物数量与总土壤酶活性相应提高。细菌数量、归一化的微生物数量与归一化的酶活性可以作为岩溶区土壤有机碳质量分数的指示指标。 |
| 英文摘要: |
| The soil organic carbon（SOC） as one of the largest and most important reservoirs of carbon（C） in the global C-cycle produces the correlated response which plays an important role in the karst region. Moreover, the relationship between SOC and microbe numbers and soil enzyme activity in karst region was evaluated to partition the soil organic carbon（SOC） accumulation mechanism. Soil samples from three karst types（saddle, depression and slop） at 0~10 cm, 10~20 cm and 20~30 cm layers in the Yaji Karst Experimental Site were collected to appraise their function and relationship. SOC contents and microbial community population were analyzed using sulfate digestion method with potassium dichromate and dilute plate incubation counting respectively. Microbial activity was valuated using soil enzyme assays as well. It could be seen that the SOC decreased from the topsoil to the fundus at three karst forms. In the 0~10 cm layer, the SOC was 40.10 g·kg-1 in depression compared to 38.28 g·kg-1 in saddle and 24.42 g·kg-1 in slop. Bacterial numbers in depression from the topsoil to the fundus were 3.26×106, 2.73×106 CFU·g-1 and 1.15×106 CFU·g-1 respectively, which are higher than that in saddle and slop. Moreover, at topsoil of 0~10 cm, urea activity was 1.92 mg·（g·24 h）-1 in depression compared to 1.12 mg·（g·24 h）-1 in saddle and 0.77 mg·（g·24 h）-1 in slop. Alkaline phosphatase activities at different layers were all higher in depression than that in same depths of addle and slop. According to the statistical analysis, the bacterial number, urea activity, normalized overall microbial number and normalized overall enzyme activity plus SOC contents had a significant positive correlation. Moreover, it could be confirmed that the soil microbial number and soil enzyme activity were improved with the accumulation of soil organic carbon. Therefore, the bacterial number, normalized overall microbial number and normalized enzyme activity could be used to indicate the soil functioning in karst region. |
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