| .铅胁迫对不同基因型谷子幼苗生理特性及基因组DNA多态性的影响[J].农业环境科学学报,2013,32(3):. |
| 铅胁迫对不同基因型谷子幼苗生理特性及基因组DNA多态性的影响 |
| Effects of Pb2+ Stress on Physiological Characteristics and DNA Polymorphism of Genome in Different Genotypes Foxtail Millet |
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| DOI：10.11654/jaes.2013.03.011 |
| 中文关键词: 谷子 Pb胁迫 生理特性 RAPD DNA多态性 |
| 英文关键词: foxtail millet[Setaria italica（L.） Beauv] Pb2+ stress physiological characteristics RAPD DNA polymorphism |
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| 摘要点击次数: 1794 |
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| 中文摘要: |
| 以4种基因型谷子为供试材料，采用盆栽土培法，研究了土壤重金属Pb2+胁迫对谷子幼苗基因组DNA多态性和可溶性糖、脯氨酸、丙二醛（MDA）含量的影响。结果表明：经50~400 mg·kg-1 Pb2+处理30 d后，4种谷子幼苗体内可溶性糖含量随Pb2+浓度上升而逐渐降低，降幅为对照的31.45%~49.11%。脯氨酸含量则表现为低浓度（≤100 mg·kg-1）的促进和高浓度（≥200 mg·kg-1）的抑制效应，MDA含量均有增加且与对照差异显著。Pb2+胁迫下不同基因型谷子幼苗基因组DNA的RAPD图谱发生明显变化，表现为单条或多条RAPD谱带的缺失、增加及荧光强度的增强或减弱，表明Pb2+明显影响谷子幼苗细胞中基因组模板DNA的稳定性，DNA多态性变化与Pb2+浓度之间存在剂量-效应关系。不同基因型谷子对Pb2+胁迫的生理和遗传损伤响应存在差异。利用RAPD技术获得的DNA多态性变化可作为检测Pb2+遗传毒性效应的生物标记物。 |
| 英文摘要: |
| Pb2+ is one of the major elements in the environment. In order to investigate the physiological characteristics and geno-toxicity effects of foxtail millet [Setaria italica（L.）Beauv] under different Pb2+ stress, four genotypes of foxtail millet（Zhaogu, Huangmi, An06, D2-8） from Shanxi, China were cultivated for 30 days in a pot filled with soil of different mass concentrations 0 mg·kg-1, 50 mg·kg-1, 100 mg·kg-1, 200 mg·kg-1, 400 mg·kg-1. Pb2+ stress effects on DNA damage of genome on foxtail millet were studied using random amplified polymorphic DNA（RAPD）, and the contents of soluble sugar, proline and MDA were tested. The results showed that the contents of soluble sugar were decreased in the range of 31.45%~49.11% compared to the control group, with the increasing of Pb2+ concentration. Also, the contents of proline synthesis were enhanced at low concentrations（less than 100 mg·kg-1）, but inhibited at high concentrations（more than 200 mg·kg-1）. The contents of MDA of four genotypes foxtail millet were significantly increased compared with the control group. The changes of random amplified polymorphic DNA profiles of four genotypes foxtail millet following Pb2+ treatment included appearance of new bands and loss of normal bands and variation of fluorescence intensity compared to the plantlet without treatment, suggesting that Pb2+ significantly affected the stability of the genomic template DNA in cells of millet seedlings. Additionally, the effect of DNA polymorphism changes was dose-dependent with the Pb2+ concentration. The physiological and genetic damage under Pb2+ stress had significant differences in the different genotypes millet. The change of DNA polymorphism using RAPD technique could be used as the biomarker to detect genotoxic effects of Pb2+. |
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