| .Mn、Zn单一及复合污染对短毛蓼氮素代谢的影响[J].农业环境科学学报,2013,32(3):. |
| Mn、Zn单一及复合污染对短毛蓼氮素代谢的影响 |
| Effects of Single Mn/Zn and Mn-Zn Combined Pollution on Nitrogen Metabolism in Polygonum pubescens Blume |
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| DOI：10.11654/jaes.2013.03.017 |
| 中文关键词: 短毛蓼 Mn-Zn复合污染 氮素代谢 |
| 英文关键词: Polygonum pubescens Blume combined pollution of Mn and Zn nitrogen metabolism |
| 基金项目: |
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| 摘要点击次数: 1953 |
| 全文下载次数: 1591 |
| 中文摘要: |
| 采用水培及正交设计的方法，研究了不同浓度Mn（0、10、100 mg·L-1）和Zn（0、10、100 mg·L-1）单一及复合处理对Mn超富集植物短毛蓼（Polygonum pubescens Bl.）生长，Mn、Zn富集能力，叶片与根硝态氮、铵态氮、游离脯氨酸、可溶性蛋白质含量及氮素代谢关键酶谷氨酸合酶（GOGAT）、谷氨酰胺合成酶（GS）、硝酸还原酶（NR）和谷氨酸脱氢酶（GDH）活性的影响。结果表明：单Zn处理时，短毛蓼株高、株重、根长及叶绿素含量均随Zn浓度的升高而降低，当Zn浓度为100 mg·L-1时，株高和株重分别为对照的78%和56%；单Mn处理对短毛蓼生长的影响不显著；Mn、Zn复合处理下，短毛蓼株高、株重、根长及叶绿素含量均随Zn浓度的升高而降低，单Zn以及单Mn处理均对短毛蓼硝态氮、铵态氮、游离脯氨酸、可溶性蛋白质含量及GOGAT、GS、NR、GDH酶的活性造成不同程度的影响。单Zn处理时，T3处理短毛蓼根及叶中GOGAT、GS、NR、GDH酶的活性较对照T1均显著（P<0.05）增强，其中根的四种酶活性分别为T1的4.47、2.72、4.17、1.88倍；单Mn处理对这四种酶活性的影响不显著；Mn、Zn复合处理降低了短毛蓼根系及叶片中硝态氮的含量（根中降低了2.60%~70.13%，叶中降低了12.67%~64.79%），同时提高了铵态氮、游离脯氨酸及可溶性蛋白质含量。在同一Zn污染水平，Mn的增加使得短毛蓼根和叶片中游离脯氨酸、可溶性蛋白质含量以及GOGAT、GS、NR、GDH酶活性均有不同程度的提高，当Zn的处理浓度为100 mg·L-1时，短毛蓼叶片中游离脯氨酸含量、GOGAT及GDH酶活性在T9处理下分别为T3的1.8、1.69、2.68倍 ，表明Mn的增加能够有效减缓Zn对短毛蓼的胁迫。 |
| 英文摘要: |
| Hydroponic culture with orthogonal design method was conducted to investigate the effects of Mn and Zn on the growth, metal accumulation and nitrogen metabolism in hyperaccumulating plants Polygonum pubescens Blume. The enzymatic activities of nitrogen metabolism were determined including nitrate reducatse（NR）, glutamine synthelase（GS）, glutamate synthase（GOGAT）, glutamate dehydrogenase（GDH） as well as the nitrate nitrogen, ammonium nitrogen and free and soluble pralines. The results showed that under single treatment of Zn, the plant height, dry weight, root length and chlorophyll content decreased as Zn concentrations increased. At 100 mg·L-1 Zn treatment, the plant height, dry weight were 78% and 56% of those under control, respectively. The growth of P. pubescens were not affected significantly when treated by Mn alone; Under combined pollution of Mn and Zn, the plant height, dry weight, root length and chlorophyll content decreased as Zn concentrations increased. Both single Zn and Mn treatment have varied impact on the contents of nitrate nitrogen, ammonium nitrogen, free prolines and soluble protein as well as enzymatic activities of GOGAT, GS, NR and GDH. Overall, Mn, and Zn com bined treatment lowered the contents of nitrate nitrogen in roots（2.60%~70.13%） and leaves（12.67%~64.79%）. But increased the contents of ammonium nitrogen, free proline and soluble protein. For the same Zn level, increased Mn addition raised the contents of free proline, and soluble protein, as well as GOGAT, GS, NR, and GDH enzymatic activities in varying degrees, indicating that Mn may effectively relieve Zn stress. |
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