| .淹水培养过程中水稻土芽孢杆菌群落结构变化特征[J].农业环境科学学报,2013,32(5):. |
| 淹水培养过程中水稻土芽孢杆菌群落结构变化特征 |
| Characteristics of Bacillus Communities in Flooded Paddy Soil |
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| DOI：10.11654/jaes.2013.05.021 |
| 中文关键词: 芽孢杆菌 群落结构 水稻土 变性梯度凝胶电泳 |
| 英文关键词: Bacillus community structure paddy soil denatured gradient gel electrophoresis（DGGE） |
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| 中文摘要: |
| 采用淹水非种植水稻土微环境模式系统，对水稻土进行1 h 和1、5、10、20、30 d 淹水培养，利用PCR-DGGE 技术检测、多元统计分析淹水培养过程中芽孢杆菌的群落结构和多样性变化规律及其影响因子。结果表明，淹水培养过程中芽孢杆菌群落结构发生了明显的演替性变化：淹水培养前期（1 h~5 d）R-策略芽孢杆菌占优势，并演替至中后期（5~30 d）；后期（10~30 d）K-对策芽孢杆菌占优势且逐渐趋于平衡。在培养初期（1 h~1 d），芽孢杆菌属微生物优势种群相似度较高且多样性指数变化较小；1~5 d期间多样性指数有较大变化；在培养后期（20~30 d），优势种群相似度最高且多样性指数趋于平衡。DCCA分析将淹水处理过程分成早期、中期和末期3类不同的生境，CCA分析进一步显示淹水过程中水稻土二价铁含量及芽孢杆菌的多样性指数H'、Ds和丰富度指数dMa变化与上述生境产生有相关性。测序结果表明，除HZ-B1外，其余9个优势DGGE 条带均属于芽孢杆菌属细菌，且大多与来自不同地域的水稻土的芽孢杆菌关系密切。 |
| 英文摘要: |
| Bacillus is one of the best characterized bacterial genera. It is defined as a gram-positive, rod-shaped bacterium which can be aerobic or facultative anaerobic. Bacillus can produce highly resistant dormant endospores in response to nutritional or environmental stresses. Since the late 19t h century, the long history of Bacilli research has included classical microbiology, biochemistry, and more modern genomic and proteomic approaches. Flooded rice field has become a model system for the study of soil microbial ecology. In this study, the total DNA was extracted from six paddy soil slurries including 1 h, 1 d, 5 d, 10 d, 20 d and 30 d of flooding incubation. The changing characteristics of community diversity and succession of Bacillus were analyzed by using Denatured Gradient Gel Electrophoresis（DGGE） based on 16S rRNA gene（rDNA） of Bacillus. The influence factors on characteristics of community diversity and succession of Bacillus in different flooding treatments were analyzed by Detrended Canonial Correspondence Analysis（DCCA） and Canonical Correspondence Analysis（CCA）. It showed that the Bacillus community structure was taken place the successive changes under different flooding times. The R-strategists organisms dominated in the early succession, while the K-strategists organisms gradually replaced them in the late succession. At the early stage of incubation（1 h~1 d）, the similarity coefficient indices of predominant Bacillus were relatively high but the variety of diversity indices were low; during 1 d and 5 d, the diversity index changed more; during the later stage of incubation（20~30 d）, there were the highest similarity coefficient indices and diversity indices kept invariableness. The flooding process could be divided into three different ecotopes including early-stage, mid-stage and later-stage, on the basis of DCCA. Further more, the CCA ordination among DGGE profiles and environmental variable factors demonstrated that Fe（Ⅱ） concentration and the changes of diversity and richness of Bacillus correlated with the above three ecotopes. From the phylogenetic tree, we found that the nine preponderant DGGE bands belonged to Bacillus except of the HZ-B1 band divided into Paenibacillus sp.. Most of them had close relations with Bacillus sp. isolated from paddy soil fields. |
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