| .天津团泊湖地区盐碱土壤中可培养微生物群落结构和归属分析[J].农业环境科学学报,2013,32(5):. |
| 天津团泊湖地区盐碱土壤中可培养微生物群落结构和归属分析 |
| Analysis of Cultivable Microbial Community Structure and Affiliation in Saline- Alkaline Soil in the Tuanbo Lake Area of Tianjin, China |
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| DOI：10.11654/jaes.2013.05.022 |
| 中文关键词: 耐盐碱细菌 16S rDNA 系统发育分析 细菌多样性 |
| 英文关键词: haloalkaliphilus 16S rDNA phylogenetic analysis bacterial diversity |
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| 中文摘要: |
| 为了解天津团泊湖地区盐碱土中可培养耐盐碱细菌的多样性，以Horikoshi Ⅱ为基础培养基，在pH 7~9.5、NaCl浓度0~15%范围内，对天津团泊湖地区盐碱土土壤样品中可培养耐盐碱细菌进行分离，并对它们进行16S rDNA序列测定和系统进化分析，共得到不同表型的细菌75株。结果表明，这些分离菌株分别属于芽孢杆菌属（Bacillus）、微小杆菌属（Exiguobacterium）、海洋芽孢杆菌属（Oceanobacillus）、鱼露芽孢杆菌属（Jeotgalibacillus）和微球菌属（Micrococcus）。芽孢杆菌属和海洋芽孢杆菌属是天津团泊湖可培养耐盐碱菌的优势细菌种群，它们分别占已测种群的48%（36株）和24%（18株）。大多数菌株与已知的系统发育关系密切的已知典型菌株之间存在一定的遗传差异（16S rDNA序列的相似性为99.6%~99.9%），其中至少有4个菌株（TJTB06、TJTB32、TJTB50和TJTB51）代表潜在的新菌株。天津团泊湖地区存在较为丰富的耐盐碱菌种群，并且潜藏着较多的新种种源，极具进一步发掘研究的价值。 |
| 英文摘要: |
| In order to reveal the diversity of cultivable haloalkaliphilus from the Tuanbo Lake Area of Tianjin, we isolated cultivable bacteria using the Horikoshi Ⅱ medium with varying NaCl concentration（0~15%） and pH（7~9.5）. A total of seventy-five halophilic bacteria were isolated from the Tuanbo Lake area. All isolated bacteria were subjected to the 16S rDNA sequence analysis. Five genera including Bacillus, Exiguobacterium, Micrococcus, Oceanobacillus, and Jeotgalibacillus were identified. Bacillus and Oceanobacillus are the dominate genera, respectively accounting for 48% and 24% of all isolated bacteria. Based on the similarity of isolated bacteria to known sequences in GenBank（16S rDNA gene sequence similartities ranged from 99.6% to 99.9%）, at least four strains（TJTB06, TJTB32, TJTB50 and TJTB51） represent potentially new strains. The results of the study showed that cultivable haloalkaliphilus bacterial populations are quite abundant in the Tuanbo Lake area. It was clear that these haloalkaliphilus have adapted to the special man-made hypersaline environment by basic physiological evolution during phylogenesis, so they could be determined according to further polyphasic taxonomy data. The current results also indicated a phenotypically and genotypically diverse culture-dependent haloalkaliphilus bacterial community colonizing the Tuanbo Lake area. They provide potentially useful microbial resources, which require further in-depth investigation. |
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