| 章 慧,郭楚玲,卢桂宁,杨 琛,党 志,吴仁人.具有产表面活性剂功能石油降解菌的筛选及其发酵条件优化[J].农业环境科学学报,2013,32(11):2185-2191. |
| 具有产表面活性剂功能石油降解菌的筛选及其发酵条件优化 |
| Screening and Fermentation Optimization of a Biosurfactant-producing Oil-degrading Bacterium |
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| DOI：10.11654/jaes.2013.11.012 |
| 中文关键词: 生物表面活性剂 原油降解菌 发酵条件优化 铜绿假单胞菌 |
| 英文关键词: biosurfactant oil-degrading bacteria fermentation optimization Pseudomonas sp.1 |
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| 摘要点击次数: 1894 |
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| 中文摘要: |
| 从广州某炼油厂附近石油污染的土壤中筛选出一株可高效产表面活性剂的原油降解菌株MZ01，结合菌株形态观察、革兰氏染色和16S rDNA 序列同源性进行分析鉴定其属于假单胞菌属（Pseudomonas sp. MZ01），该菌9 d对原油的降解率达54.7%。通过正交实验优化其产表面活性剂的环境因子并进行发酵培养，结果表明，MZ01最佳发酵条件为：酵母膏（3 g·L-1）作为氮源，玉米油（2 g·L-1）作为碳源，温度为25 ℃，pH值为9.0和含盐量为5%。该条件下3 d的发酵产物经提纯后得到表面活性剂产量为2.27 g·L-1，测得该产物CMC值为0.1 g·L-1，可将水的表面张力从初始的72 mN·m-1降至30 mN·m-1。 |
| 英文摘要: |
| The aim of the present study was to isolate oil-degrading bacterial strains with high ability to produce biosurfactants. Bacterial strains from oil contaminated soil near a refinery were enriched and incubated with oil as the sole carbon source. Bacterial strains to produce biosurfactants were then screened by testing oil collapse-dropping, emulsification index and surface tension, and those strains with the ability to degrade oil were further screened by DCPIP tests. An oil-degrading bacterial strain MZ01 with high ability to produce biosurfactants was obtained. It was identified as Pseudomonas sp. MZ01 by gram-staining, morphology, and 16S rDNA sequencing. MZ01 could degrade crude oil by 54.7% within 9 days. The optimal conditions for MZ01 to produce biosurfactants were explored by orthogonal experiments. These conditions were yeast extract（3 g·L-1） as nitrogen source, corn oil（2 g·L-1） as carbon source, initial pH 9.0, 5% salinity and temperature 25 ℃. The production of biosurfactants by MZ01 was 2.27 g·L-1 after 3-day fermentation. The critical micelle concentration（CMC） of the biosurfactants was 0.1 g·L-1, reducing the surface tension of pure water from 72 mN·m-1 to 30 mN·m-1. |
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