文章摘要
陈爱梅,王金花,夏晓明,王 娟,朱鲁生,范燕燕.不同剂型吡虫啉对蚯蚓和斑马鱼的急性毒性评价[J].农业环境科学学报,2013,32(9):1758-1763.
不同剂型吡虫啉对蚯蚓和斑马鱼的急性毒性评价
Acute Toxicity of Imidacloprid with Different Formulation on Earthworm and Zebrafish
  
DOI:10.11654/jaes.2013.09.008
中文关键词: 吡虫啉  剂型  蚯蚓  斑马鱼  急性毒性
英文关键词: imidacloprid  formulation  earthworm(Eisenia foetida)  zebrafish  acute toxicity
基金项目:
作者单位
陈爱梅 山东农业大学资源与环境学院植物保护学院 农药环境毒理研究中心 山东省高校农业环境重点实验室 
王金花 山东农业大学资源与环境学院植物保护学院 农药环境毒理研究中心 山东省高校农业环境重点实验室 
夏晓明 山东农业大学资源与环境学院植物保护学院 农药环境毒理研究中心 山东省高校农业环境重点实验室 
王 娟 山东农业大学资源与环境学院植物保护学院 农药环境毒理研究中心 山东省高校农业环境重点实验室 
朱鲁生 山东农业大学资源与环境学院植物保护学院 农药环境毒理研究中心 山东省高校农业环境重点实验室 
范燕燕 扬州大学环境科学与工程学院 
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中文摘要:
      为了评价农药不同剂型对环境生物的毒性影响,利用人工土壤法和静态水鱼类毒性测试法研究了不同剂型吡虫啉对蚯蚓和斑马鱼的急性毒性效应。结果表明:35%吡虫啉悬浮剂、70%吡虫啉湿拌种剂、70%吡虫啉可湿性粉剂、600 g·L-1吡虫啉悬浮种衣剂对蚯蚓7 d LC50值与95%置信限(见括号)分别为:0.82(0.62~0.89),6.44(5.17~7.83),2.39(2.10~2.61),9.65(7.79~11.77)a.i.mg·kg-1干土;14 d LC50值为0.77(0.59~0.83),6.00(4.34~6.79),2.12(1.62~2.33),8.60(0.23~10.19)a.i.mg·kg-1干土。吡虫啉对斑马鱼的急性毒性结果表明:70%吡虫啉湿拌种剂对鱼的24、48、72、96 h的LC50值分别为24.3(16.3~28.8),22.8(13.0~27.7),20.1(4.0~27.4),17.8(2.4~26.2)a.i.mg·L-1;70%吡虫啉可湿性粉剂对鱼不同处理时间的LC50值分别为46.4(41.6~48.0),45.7(38.4~47.6),45.2(36.2~47.2),45.2(36.2~47.2)a.i.mg·L-1; 35%吡虫啉悬浮剂和600 g·L-1吡虫啉悬浮种衣剂对鱼不同处理时间LC50值均大于1.00×102 a.i.mg·L-1。依据《化学农药环境安全评价试验准则》,35%吡虫啉悬浮剂对蚯蚓的毒性为高毒,其他3种为中毒;4种剂型吡虫啉对斑马鱼的毒性均为低毒。
英文摘要:
      An acute toxicity study was conducted to evaluate the ecological risk of pesticide with different formulations on environmental organisms by artificial soil test and static test. Results of artificial soil test showed that LC50 values of 35% imidacloprid aqueous suspension concentrate(SC), 70% imidacloprid wet seed dressing agent(WS), 70% imidacloprid wettable powder(WP), and 600 g·L-1 imidacloprid flowable concentrate for seed coating(FS) for earthworm in 7 d were 0.82(0.62~0.89), 6.44(5.17~7.83), 2.39(2.10~2.61) and 9.65(7.79~11.77)a.i.mg·kg-1, respectively. Corresponding LC50 values in 14 d were 0.77(0.59~0.83), 6.00(4.34~6.79), 2.12(1.62~2.33) and 8.60(0.23~10.19) a.i.mg·kg-1, respectively. Results of a static test showed that the LC50 values of 70% imidacloprid WS for zebrafish on 24 h, 48 h, 72 h, and 96 h were 24.3(16.3~28.8), 22.8(13.0~27.7), 20.1(4.0~27.4) and 17.8(2.4~26.2) a.i.mg·L-1, respectively. LC50 values of 70% imidacloprid WP for zebrafish were 46.4(41.6~48.0), 45.7(38.4~47.6), 45.2(36.2~47.2) and 45.2(36.2~47.2) a.i.mg·L-1, respectively. LC50 values of both 35% imidacloprid SC and 600 g·L-1 imidacloprid FS for zebrafish were higher than 1.00×102 a.i.mg·L-1. According to the Experimental Criterion for Environmental Safety Assessment of Chemical Pesticide, the toxic effect of 35% imidacloprid SC on earthworms was significant, while other formulations of imidacloprid were moderate. In additional, the toxic effects of all different formulations of imidacloprid on zebrafish were not serious.
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