| 刘根林,戚金亮,喻德跃,杨永华.根际箱种植富含硫氨基酸转基因大豆对土壤硫转化酶活性及微生物功能多样性的影响[J].农业环境科学学报,2014,33(1):103-110. |
| 根际箱种植富含硫氨基酸转基因大豆对土壤硫转化酶活性及微生物功能多样性的影响 |
| Effects of Sulfur Amino Acids-rich Transgenic Soybean on Sulfur Transforming Enzyme Activity and Microbial Functional Diversity in Soil |
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| DOI：10.11654/jaes.2014.01.013 |
| 中文关键词: 土壤 硫 芳基硫酸酯酶 微生物 功能多样性 |
| 英文关键词: soil sulfur arylsulfatase microbe functional diversity |
| 基金项目: |
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| 摘要点击次数: 3397 |
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| 中文摘要: |
| 为研究转基因作物的土壤生态安全性，于根际箱中种植2组大豆（A组：转基因品系OE-8、OE-7、RNAi-3和受体南农88-1；B组：转基因品系Gaga1 17-4、Gaga1 21-8、Gaga1 57和受体N2899），采集大豆花期根际土壤，分析硫元素含量和芳基硫酸酯酶活性，并采用Biolog微平板法，研究相比于受体，转基因品系对土壤微生物群落功能多样性的影响。结果表明：OE-8和RNAi-3与其受体相比，根际土壤硫元素含量显著降低；2组组内对比结果显示，根际土样芳基硫酸酯酶活性差异均不显著。Biolog GN/GP/FF/ECO系统及多样性指数分析显示：A组中RNAi-3根际土壤微生物群落活性显著低于受体，微生物群落的丰富度、多样性和均度显著降低，OE-7根际土壤微生物群落的丰富度和均度显著降低；B组中Gaga1 57和Gaga1 17-4根际土壤微生物群落的丰富度、多样性和均度显著下降。 |
| 英文摘要: |
| Growing transgenic crops may have ecological consequences. In order to evaluate soil ecological risks of transgenic crops, a rhizo-box experiment was carried out to examine sulfur content, arylsulfatase activity, and microbial functional diversities in the rhizospheric soils grown with two groups of soybean：Group A（transgenic lines OE-8, OE-7, RNAi-3 and their recipient Nannong 88-1 as CK-1） and Group B（transgenic lines Gaga1 17-4, Gaga1 21-8, Gaga1 57 and their recipient N2899 as CK-2）. At florescence stage, the content of sulfur in the rhizospheric soils of OE-8 and RNAi-3 was significantly lower than that of CK. Arylsulfatase activity had no significant difference between transgenic lines and their CKs. The Biolog analysis（GN/GP/FF/ECO systems） showed that the average well color developments（AWCDs） of rhizospheric soil of Line RNAi-3 in the Group A were significantly lower than the control, illustrating that planting Line RNAi-3 inhibited the physiology of soil Gram-negative（G－） bacteria, Gram-positive（G+） bacteria and fungi. However, in Group B the AWCDs values of Gaga1 21-8 significantly increased, implying that planting Line Gagal 21-8 stimulated the physiology of soil G+ bacteria. Compared with the control, the richness, diversity and evenness of soil microbial community in Line RNAi-3 were all significantly lower; and the richness and evenness in Line OE-7 were also significantly declined. Significant reduction in the richness, diversity and evenness in both Line Gaga1 57 and Line Gaga1 17-4 was observed. It is concluded that growing transgenic soybeans has negative impacts on soil microbial activities and microbial functional diversity in rhizospheric soils. |
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