侧孢芽孢杆菌对铜绿微囊藻生长胁迫的研究

王 琼; 吴 伟; 季 丽

文章摘要

王琼,吴伟,季丽.侧孢芽孢杆菌对铜绿微囊藻生长胁迫的研究[J].农业环境科学学报,2014,33(2):383-391.

侧孢芽孢杆菌对铜绿微囊藻生长胁迫的研究

Influence of Bacillus laterosporus on Microcystis aeruginosa Growth

DOI：10.11654/jaes.2014.02.025

中文关键词: 侧孢芽孢杆菌铜绿微囊藻共适培养基胁迫无机氮

英文关键词: Bacillus laterosporus Microcystis aeruginosa co-substrates stress inorganic nitrogen

基金项目:

作者	单位
王琼	南京农业大学无锡渔业学院，江苏无锡 214081
吴伟	南京农业大学无锡渔业学院，江苏无锡 214081；中国水产科学研究院淡水渔业研究中心，中国水产科学研究院内陆渔业生态环境与资源重点开放实验室，江苏无锡 214081
季丽	南京农业大学无锡渔业学院，江苏无锡 214081

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中文摘要:

为探讨侧孢芽孢杆菌（Bacillus laterosporus）SK-1对铜绿微囊藻（Microcystis aeruginosa）生长胁迫的影响，在实验室环境下对两者共适培养基质进行了选择和优化，探究了处于不同生长期和不同初始生物量的上述两者在共基质中的数量消长规律，以及共基质体系中无机氮营养盐（NO-3-N、NO-2-N、NH+4-N）的含量变化。结果显示，将取自富营养化池塘的养殖水，添加适量葡萄糖和氨氮使得C∶N∶P的近似比为250∶10∶1的培养基质作为共适培养基质。在此基质中，不同生长时期的侧孢芽孢杆菌与铜绿微囊藻共存时的生长胁迫效应不同，处于生长稳定期的菌体对生长初期的微囊藻抑制效果最好，且在一定的浓度范围内，菌体细胞密度越大，其抑藻效果越明显。研究表明，侧孢芽孢杆菌对铜绿微囊藻生长的抑制是通过竞争营养物质与分泌胞外抑藻物质这两种方式实现的，且在藻体和菌体的不同生长阶段，这两种作用方式所占的比重不同。故在生态控藻时，须在藻类爆发生长前进行细菌种群的培育，以获得良好的生态效果。

英文摘要:

Interactions of algae and algae-inhibiting bacteria often control the stability of aquatic ecosystems. In this study the influence of Bacillus laterosporus SK-1 on the growth of Microcystis aeruginosa was examined under different initial biomass and at different growing periods. Concentrations of inorganic nitrogen（NO-3-N, NO-2-N, NH+4-N） in the system were also monitored. A growth media with 250∶10∶1 of C∶N∶P ratio obtained by adding glucose and ammonia nitrogen to eutrophic water was used to grow Bacillus laterosporus and Microcystis aeruginosa. The bacteria had different stresses on the growth of Microcystis aeruginosa at different growth stages. The strain SK-1 at its stable stage had the greatest inhibitory effect on Microcystis aeruginosa of early growth stages. The more the initial SK-1 amount, the greater algae-inhibiting effect. The strain SK-1 inhibited the growth of Microcystis aeruginosa mainly through competing nutrients and secreting extracellular algae-inhibiting substances. The relative proportion of these two processes were different at different growth stages of algae and bacteria. The results suggest that bacteria should be inoculated prior to algae outbreak to best control algae ecologically.

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