| 苏银萍,刘 华,于方明,李 艺,陈孟林,周振明,李明顺.Mn污染对木荷叶片抗氧化酶系统的影响[J].农业环境科学学报,2014,33(4):680-686. |
| Mn污染对木荷叶片抗氧化酶系统的影响 |
| Effects of Manganese Contamination on Activities of Antioxidant Enzyme Systems in Leaves of Schima superba |
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| DOI：10.11654/jaes.2014.04.009 |
| 中文关键词: Mn污染 木荷 抗氧化酶系统 |
| 英文关键词: manganese contamination Schima superba activity of antioxidant enzyme system |
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| 摘要点击次数: 1868 |
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| 中文摘要: |
| 采用土培方法，研究了广西桂林市未受污染土壤（对照）及贺州市某锰矿的未开采区、探矿区、恢复区、采矿区土壤和尾砂对Ｍｎ富集植物木荷（Ｓｃｈｉｍａ ｓｕｐｅｒｂａ）生长、Ｍｎ吸收，叶片超氧化物歧化酶（ＳＯＤ）、过氧化氢酶（ＣＡＴ）、过氧化物酶（ＰＯＤ）、抗坏血酸过氧化物酶（ＡＰＸ）活性，抗坏血酸（ＡｓＡ）、谷胱甘肽（ＧＳＨ）、植物络合素（ＰＣｓ）、巯基（－ＳＨ）、丙二醛（ＭＤＡ）、过氧化氢（Ｈ２Ｏ２）含量，以及超氧阴离子自由基（Ｏ－2·）产生速率的影响。结果表明，随着土壤Ｍｎ含量的增加，木荷根、茎、叶中Ｍｎ含量均显著增加（P＜０．０５），株高及株重呈先上升后下降的趋势，表明一定程度的Ｍｎ能促进木荷的生长。土壤Ｍｎ含量的增加，使得叶片中Ｏ－2·的产生速率及Ｈ２Ｏ２的含量呈现不同程度的变化，采矿区土壤栽培的木荷叶片中Ｏ－2·的产生速率显著高于对照（P＜０．０５），开采区土壤和尾砂栽培的木荷叶片中Ｈ２Ｏ２含量比对照分别提高了６５．７％、９６．３％，矿区土壤栽培的木荷叶片中ＭＤＡ的含量均显著高于对照（P＜０．０５），表明叶片受到一定的胁迫。木荷叶片中抗氧化酶活性与非酶物质变化不尽相同，ＳＯＤ、ＰＯＤ酶活性、ＡｓＡ、ＧＳＨ含量均随着土壤Ｍｎ浓度的增加而增加，ＣＡＴ、ＡＰＸ酶的活性呈先升后降趋势，其中尾砂栽培的木荷ＰＯＤ酶的活性最高，比对照提高了１．62倍，采矿区土壤和尾砂栽培的木荷GSH的含量显著高于对照（P<0.05)，分别比对照提高了1.66和1.79倍。 |
| 英文摘要: |
| Manganese（Mn） is essential for plant growth, but excess Mn may impact antioxidant enzyme systems in plant. A soil culture experiment was performed to explore the effects of Mn contamination on various enzymatic antioxidants and non-enzymatic antioxidants in the leaves of Schima superba. Soil samples, collected from unmining section（WK）, prospecting area（T）, restored area（H）, mining（K） and tailings（W） in a manganese mine of Hezhou, were used as Mn-polluted soils, and an unpolluted soil from Guilin, Guangxi was used as control（CK）. After 145-d of culture, leaves of S. superba were sampled and analyzed for the activities of superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, and ascorbate peroxidase（APX） and the contents of ascorbic acid（AsA）, glutathione（GSH），phytochelatins（PCs），total acid soluble SH，malondialdehyde（MDA）, hydrogen peroxide（H2O2）, and the produce rate of superoxide anion（Ｏ－2·）. Concentrations of Mn in roots, stems, and leaves increased with soil Mn concentrations. Compared to the control, plant biomass increased in WK and T samples, whereas decreased in the other soils, indicating stimulation of S. superba growth by Mn at low concentrations. The rate of Ｏ－2·production in the leaves was significantly higher in H than in control（P<0.05）. The contents of H2O2 in leaves were increased by 65.7% and 96.3% for K and W, respectively. The leaf MDA was significantly（P<0.05） higher in mineral soils than in control, showing that the leaves were damaged by Mn pollution. The activities or contents of SOD, POD, AsA and GSH increased in order of CK |
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