| 龙 瑞,张 明,张光生,成小英.Mn、Cd单一及其复合胁迫对伊乐藻生长及生理生化的影响[J].农业环境科学学报,2014,33(6):1112-1117. |
| Mn、Cd单一及其复合胁迫对伊乐藻生长及生理生化的影响 |
| Effects of Mn and Cd and Their Combination on Growth and Physiological and Biochemical Characteristics of Elodea nutttallii |
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| DOI：10.11654/jaes.2014.06.009 |
| 中文关键词: Mn2+ Cd2+ 复合胁迫 伊乐藻 生长 生理生化 |
| 英文关键词: Mn2+ Cd2+ combined stress Elodea nutttallii growth physiological and biochemical characteristics |
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| 摘要点击次数: 1910 |
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| 中文摘要: |
| 以不同浓度Mn2+、Cd2+单一及其复合胁迫处理7 d的伊乐藻为实验材料，研究了Mn2+、Cd2+单一及其复合处理对伊乐藻叶片叶绿素、可溶性蛋白、丙二醛（MDA）、过氧化氢（H2O2）、超氧阴离子（O-2·）含量以及抗氧化酶[超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）]活性的影响。结果表明，低浓度的Mn2+（≤1 mg·L-1）能够促进叶绿素和可溶性蛋白的合成，刺激MDA、H2O2、O-2·的生成，抗氧化酶活性也逐步上升；高浓度时，叶绿素和可溶性蛋白含量开始减少，MDA、H2O2、O-2·含量仍继续上升，伊乐藻叶片内酶活性机制受到不同程度的抑制，SOD继续上升，POD、CAT开始下降。Cd2+胁迫下，叶绿素和可溶性蛋白的含量随着浓度的增加而降低，呈明显的剂量-效应关系，MDA、H2O2、O-2·含量均显著增加，抗氧化酶活性均随着Cd2+浓度呈先上升后下降的趋势，Cd2+浓度0.01 mg·L-1时CAT活性达到最大，1 mg·L-1时SOD、POD活性达到最大。Cd2+对植物生理生化的影响较Mn2+大，伊乐藻对Cd2+污染的抵御能力较差。高浓度重金属胁迫时，SOD起主要的保护作用，POD和CAT的保护作用相对降低。Mn2+和Cd2+复合胁迫较单一胁迫对伊乐藻的影响大，Mn2+含量较高时表现为协同作用。 |
| 英文摘要: |
| Elodea nutttallii, an aquatic plant, has showed potential for ecological restoration of polluted water. Here, we examined chlorophyll, soluble protein, malondialdehyde（MDA）, hydrogen peroxide（H2O2）, and superoxide anion（O-2·）contents, and superoxide dismutase（SOD）, peroxidase（POD）and catalase（CAT））activities in Elodea nutttallii under different Mn2+ and Cd2+ stresses for 7 days. Low concentrations of Mn2+ promoted contents of chlorophyll, soluble protein, MDA, H2O2 and O-2·, and stimulated activities of antioxidant enzymes. Under high concentrations of Mn2+, the chlorophyll and soluble protein contents and POD and CAT activities decreased, while MDA, H2O2 and O-2· contents and SOD activity increased. Under Cd2+ stresses, the chlorophyll and soluble protein contents decreased, showing a dose-response relationship; whereas MDA, H2O2 and O-2· significantly increased the activities of antioxidant enzymes at low Cd2+ stresses but decreased at high Cd2+ concentrations. The CAT activity was greatest at 0.01 mg Cd·L-1, while the activities of SOD and POD were highest at 1 mg Cd·L-1. Compared with POD and CAT, SOD played greater protection under high concentrations of heavy metals. The combined effects of Mn2+ and Cd2+ on the plant were greater than their single. The synergy effects of Mn2+ and Cd2+ were observed under the high Mn2+ concentrations. In conclusion, Cd has greater stresses than Mn2+ on the physiological and biochemical characteristics of the plant. Elodea nutttallii shows poorer resistance to Cd2+ than Mn2+ stresses. |
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