| 李文婷,裘丽萍,李志波,李丹丹,宋 超,陈家长.甲萘威暴露对雄性罗非鱼血清中内分泌相关酶活性的影响[J].农业环境科学学报,2014,33(7):1304-1309. |
| 甲萘威暴露对雄性罗非鱼血清中内分泌相关酶活性的影响 |
| Effects of Carbaryl on Endocrine System in Serum of Nile Tilapia（GIFT Oreochromis niloticus） |
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| DOI：10.11654/jaes.2014.07.007 |
| 中文关键词: 甲萘威 罗非鱼 内分泌干扰 |
| 英文关键词: carbaryl Nile tilapia endocrine disruption |
| 基金项目: |
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| 摘要点击次数: 1712 |
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| 中文摘要: |
| 实验室条件下，尼罗罗非鱼暴露于不同浓度的甲萘威（0.16、0.8、2 mg·L-1）下29 d，通过酶联免疫法测定黄体生成素（LH）、卵泡刺激素（FSH）、三碘甲状腺原氨酸（T3）、甲状腺素（T4）水平变化，探讨甲萘威对尼罗罗非鱼内分泌干扰效应。研究结果显示，在实验期间与对照组相比，0.16、0.8、2 mg·L-1甲萘威暴露下的LH、FSH、T3、T4水平均随暴露时间增加不断升高，其中：10~19 d内上升幅度小，20~29 d内上升幅度较大，在实验第29 d，LH、FSH、T3、T4水平均达到暴露以来的最大值。研究表明，从甲萘威对鱼体存在10 d的短期积累效应来看，0.8 mg·L-1的甲萘威具有最强的内分泌干扰能力，此浓度下的LH、FSH、T3、T4水平在暴露第10 d均高于其他两个浓度组；从29 d的长期积累效应来看，2 mg·L-1浓度组的各激素水平上升幅度最大。 |
| 英文摘要: |
| Carbaryl, the worldwide most used carbamate insecticide, has not only the neurotoxicological effects via inhibiting the activity of acetylcholinesterase, but also the endocrine disrupt effects. Under laboratory conditions, Nile tilapias were exposed to different concentrations of carbaryl（0.16, 0.8, and 2 mg·L-1） for 29 days to examine the interfering effects of carbaryl on their endocrine system. Enzyme-linked immunosorbent assay was used to determine the levels of luteinizing hormone（LH）, follicle-stimulating hormone（FSH）, triiodothyronine（T3） and thyroxine（T4）. Compared with the control, the LH, FSH, T3 and T4 levels under all carbaryl concentrations（0.16 mg·L-1, 0.8 mg·L-1 and 2 mg·L-1） increased gradually with increasing exposure time, greater increases occurred between the 20th to 29th days. During the short-term exposure（<10 days）, however, carbaryl had the strongest endocrine disruption at 0.8 mg·L-1. The LH, FSH, T3 and T4 levels were higher at this concentration than other concentrations. During the long-term exposure（29 days）, however, all hormone levels were the greatest at 2 mg·L-1 carbaryl. These results would advance understanding of the effects of carbamates insecticides on the reproduction, physiology, and neuroendocrine immune of tilapia. |
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