| 郭彦钊,付瑞敏,薛婷婷,谷亚楠,马玮超,陈五岭.二甲基硫醚氧化菌的分离鉴定及其对猪粪堆肥的影响[J].农业环境科学学报,2016,35(2):372-379. |
| 二甲基硫醚氧化菌的分离鉴定及其对猪粪堆肥的影响 |
| Isolation and identification of a DMS-oxidizing bacterium and its impacts on swine manure composting |
| 投稿时间：2015-07-28 |
| DOI：10.11654/jaes.2016.02.023 |
| 中文关键词: 二甲基硫醚 那不勒斯硫杆菌 16S rDNA 猪粪堆肥 酶活 |
| 英文关键词: dimethyl sulfide Thiobacillus neapolitanus 16S rDNA swine manure composting enzyme activity |
| 基金项目:农业部科技成果与转化项目(2012GB2 G000451.);陕西省重大科技创新项目(2009ZKC04-16) |
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| 中文摘要: |
| 从污水处理厂活性污泥中分离得到一株二甲基硫醚(DMS)氧化菌AM2,经16S rDNA检测鉴定其为那不勒斯硫杆菌(Thiobacillus neapolitanus)。AM2的最适生长温度为30℃,最适pH为7.0。以猪粪和秸秆粉为原料进行肥料堆肥试验,通过检验菌株AM2对堆肥过程中DMS排放量、堆肥温度、可培养微生物和酶活性的影响,来探究菌株AM2对堆肥的影响。结果表明:接入菌株AM2的实验组DMS释放量显著(P<0.05)少于对照组,在30 d的实验中有20 d减少60%以上;显著提高了(P<0.05)可培养微生物数量,同时将物料温度高于50℃的天数延长至13 d;实验各组的脲酶、脱氢酶、过氧化氢酶、蛋白酶和纤维素酶酶活都显著高于对照组。综合判断,菌株AM2能够有效降低堆肥过程中DMS的排放量并促进肥料腐熟。 |
| 英文摘要: |
| A DMS-oxidizing bacterial strain AM2 was isolated from activated sludge by using streak plate method, and was identified as Thiobacillus neapolitanus. Its optimum temperature and pH were respectively 30℃ and 7.0. Impact of the strain AM2 on swine and straw mixture composting was examined by monitoring DMS outputs, temperature, culturable microorganism population and enzyme activity in the compost. Compared with control, inoculation with AM2 not only significantly(P<0.05) decreased DMS outputs with over 60% reduction for 20 days during 30-day experimental period, but also significantly(P<0.05) promoted culturable microbe population. Compost temperature maintained above 50℃ for 13 days during the experimental period. Activities of dehydrogenase, urease, catalase, protease and cellulose were significantly(P<0.05) higher in DMS-inoculated than in control groups. To sum up, the strain AM2 can effectively reduce DMS emissions during composting process and promote compost maturity. |
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