钒胁迫下枸杞和芥菜幼苗蛋白的分子分布研究

侯明; 赵军平; 李明沅; 甘焕辉

文章摘要

侯明,赵军平,李明沅,甘焕辉.钒胁迫下枸杞和芥菜幼苗蛋白的分子分布研究[J].农业环境科学学报,2016,35(4):634-639.

钒胁迫下枸杞和芥菜幼苗蛋白的分子分布研究

Distribution of protein molecular in seedlings of Lycium barbarum and Brassica juncea under vanadiumstresses

投稿时间：2015-11-15

DOI：10.11654/jaes.2016.04.004

中文关键词: 钒胁迫枸杞芥菜分子分布蛋白质

英文关键词: vanadium stress Lycium barbarum Brassica juncea molecular distribution protein

基金项目:国家自然科学基金项目(41161076,41561077)

作者	单位
侯明	桂林理工大学化学与生物工程学院, 广西桂林 541004
赵军平	桂林理工大学化学与生物工程学院, 广西桂林 541004
李明沅	桂林理工大学化学与生物工程学院, 广西桂林 541004
甘焕辉	桂林理工大学化学与生物工程学院, 广西桂林 541004

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中文摘要:

通过G-75凝胶层析结合电泳分析,研究了不同V(吁)浓度胁迫下枸杞和芥菜的蛋白分子分布。经过凝胶层析后出现两个紫外吸收峰,第一峰主要集中在8号至15号管,第二峰集中在25号至33号管。对第一紫外吸收峰的电泳分析表明,V胁迫下植物蛋白的分子分布发生了变化:当V浓度为10 mg·L^-1时,枸杞根产生了分子量为58.6 KD蛋白,茎产生了分子量分别为64.5、46.9KD两种蛋白;当V浓度增大到20 mg·L^-1,叶产生了分子量为20 KD蛋白。随着V胁迫浓度增大,芥菜中分子量为33.9 KD和36KD两条蛋白条带逐渐变浅,而27.3 KD蛋白条带逐渐加深。枸杞幼苗第一洗脱峰蛋白中V含量随V胁迫浓度增大而增加,而芥菜叶在V≥10 mg·L^-1时V含量降低。这与不同植物蛋白结合V的能力有关,因枸杞幼苗蛋白结合V的能力较强,生成的植物螯合肽较多,故对V有较强的耐受能力。

英文摘要:

Protein molecular distribution in Lycium barbarum and Brassica juncea under different vanadium(V) stress levels was studied by G-75 gel chromatography and electrophoresis analysis. After gel chromatography, two UV peaks appeared, with the first peak occurring in No.8~No.15 tubes, and the second peak in No.25~No.33 tubes. Results of electrophoresis analysis from the first UV peak indicated that the molecular weight distribution changed under the V stresses. At 10 mg V·L^-1, Lycium barbarum produced a kind of protein with molecular weight of 58.6 KD in roots, while in stems two kinds of protein with molecular weight of 64.5 and 46.9 KD. At 20 mg V·L^-1, the leaves produced a kind of protein with molecular weight of 20 KD. With V stress levels increasing, two protein bands with 33.9 KD and 36 KD in Brassica juncea gradually changed to shallow, but that with 27.3 KD was deepened progressively. The V concentrations in the protein of the first elution peak in Lycium barbarum seedlings increased with increasing V stress levels, while the V concentrations in leaves of Brassica juncea decreased when V stress levels ≥10 mg·L^-1. This may be due to differences in V-binding capacity of different plant proteins. The V-binding ability of Lycium barbarum seedlings was greater than that of Brassica juncea, and thus more phytochelatins were generated, resulting in stronger tolerance of Lycium barbarum seedlings to V.

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