| 李翠,温海峰,郑瑞伦,陈洁,马雪菊,王庆海.阿特拉津胁迫对菖蒲的生理毒性效应[J].农业环境科学学报,2016,35(10):1895-1902. |
| 阿特拉津胁迫对菖蒲的生理毒性效应 |
| Phytotoxicity of atrazine to Acorus calamus L. |
| 投稿时间：2016-03-09 |
| DOI：10.11654/jaes.2016-0301 |
| 中文关键词: 阿特拉津 菖蒲 生理特性 水污染 抑菌条件 耐受性 |
| 英文关键词: atrazine Acorus calamus L. physiological index water pollution sterile condition tolerance |
| 基金项目:国家自然科学基金项目（31370540） |
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| 中文摘要: |
| 为明确阿特拉津对菖蒲的毒性效应，本文通过水培实验研究了阿特拉津浓度和培养时间在抑菌和不抑菌条件下对菖蒲叶绿素含量、叶绿素a/b值、丙二醛含量（MDA）、抗氧化酶（SOD和POD）活性和最大光能转化效率的影响。结果表明：不抑菌条件下，叶绿素a和叶绿素总量随处理浓度的增加和培养时间的延长逐渐降低，叶绿素a/b值无显著变化；MDA含量随着培养时间延长呈现先升高后降低的趋势，至培养第5周，≤2 mg·L-1处理的MDA含量恢复至对照水平；SOD和POD活性随培养时间延长和处理浓度增加均无显著变化；最大光能转化效率（Fv/Fm）随处理浓度的增加而降低，0.5 mg·L-1处理与对照无显著差异，≥1 mg·L-1处理培养1~4周显著低于对照，第5周时恢复至对照水平。抑菌条件下，培养第1周叶绿素含量和Fv/Fm均显著低于不抑菌处理，培养4~5周时，≥1 mg·L-1处理Fv/Fm亦显著低于不抑菌处理；整个试验期间≥2 mg·L-1处理Fv/Fm均显著低于对照水平。可见，菖蒲对阿特拉津胁迫具有较好的耐受能力，水培系统中的微生物可在一定程度上减轻阿特拉津胁迫对菖蒲的毒性效应。 |
| 英文摘要: |
| Long term applications of atrazine have resulted in water and soil contamination. In this study, a hydroponic experiment was designed to assess the toxicity of atrazine to emergent macrophyte Acorus calamus L., by measuring chlorophyll content, chlorophyll a/b ratio, maximal photochemical efficiency(Fv/Fm), malondialdehyde(MDA) content, peroxidase(POD), and superoxide dismutase(SOD) activities under unsterile and sterile conditions. In unsterile treatment, plant chlorophyll content in leaves reduced with increases in atrazine concentrations and cultivation time. Chlorophyll a/b ratio had no significant difference compared with that of the control during the entire cultivation period. MDA content increased initially and then decreased with the prolongation of cultivate time. After 5-week exposure, MDA content had no significant differences between atrazine treatment groups at concentrations less than 2 mg·L-1, as compared with control. Fv/Fm ratio reduced with increasing atrazine concentrations, but no significant difference was found between 0.5 mg·L-1 treatment and the control. It, however, recovered to the control's level after the 5th week exposure. Under sterile condition, chlorophyll content and Fv/Fm decreased significantly compared with those in unsterile condition within 1-week exposure. Such significant difference in Fv/Fm still existed at atrazine level above 1 mg·L-1 under 4- and 5-week exposures. During the whole experimental period, Fv/Fm ratio was significantly lower in atrazine treatments than in the control when atrazine concentration exceeded 2 mg·L-1 under sterile condition. Acorus calamus L. was highly tolerant to atrazine stress and microorganism in the hydrophic system, to a certain extent, can alleviate the phytotoxicity of atrazine stress. |
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