| 张松,侯彬,纪婷婷,刘阳,黄飞,秦梓菲,卢静.生物质炭固定化融合菌株F14方法的研究及其对芘的去除[J].农业环境科学学报,2018,37(3):464-470. |
| 生物质炭固定化融合菌株F14方法的研究及其对芘的去除 |
| Degradation of pyrene by immobilized cells of fusant bacterial strain F14 using biochar |
| 投稿时间：2017-09-29 |
| DOI：10.11654/jaes.2017-1337 |
| 中文关键词: 融合菌株 固定化微生物 生物质炭 多环芳烃 |
| 英文关键词: fusant bacterial strain immobilized microorganism biochar PAHs |
| 基金项目:国家自然科学基金青年基金项目（41401355）；山西省青年科技研究基金项目（2015021053）；广东省自然科学基金项目（2014A030310320） |
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| 摘要点击次数: 2069 |
| 全文下载次数: 2034 |
| 中文摘要: |
| F14是一株用原生质体融合技术构建的能够降解多环芳烃（PAHs）的融合菌株，利用生物质炭固定化F14制备固定化微生物小球来提高该菌株对芘的去除能力。实验在确定聚乙烯醇（PVA）和海藻酸钠（SA）最佳组合比例的基础上，研究了不同温度下（300、500℃和700℃）制备的生物质炭形成的固定化小球的性能以及对芘的去除效果。结果表明：制备的固定化小球的直径约为3 mm，对固定化小球成球效果、传质性能和机械性能的分析表明，载体PVA和SA的最佳组合比例为8%和1%；对不同温度下制备的生物质炭的固定化小球的传质性能和扫描电镜图进行分析，表明700℃制备的生物质炭的固定化小球性能较好。与游离菌相比，固定化小球对芘的去除能力更高且速率更快，对初始浓度为50 mg·L-1的芘10 d内去除率高达94.91%，比游离菌对芘的去除率高了近58%，同时固定化小球可以重复使用高达9次。 |
| 英文摘要: |
| F14 is a fusant strain constructed by protoplast fusion and is able to degrade polycyclic aromatic hydrocarbons(PAHs). To improve the pyrene removal rate, F14 was immobilized using biochar. The removal of pyrene by immobilized beads with added biochar produced at different temperatures(300, 500℃, and 700℃) was investigated, based on a study of the optimum combination ratio of polyvinyl alcohol(PVA) and sodium alginate(SA). The results showed that the diameter of the immobilized beads was about 3 mm, and an analysis of the effects, mass transfer, and mechanical properties of the immobilized beads showed that the best ratios of PVA and SA were 8% and 1%, respectively. Further analysis of mass transfer and scanning electron microscopy of the immobilized beads made by different biochars showed that the immobilized beads made from 700℃ biochar had better performance. Immobilized cells presented a higher and faster removal ability than free cells for pyrene, and 94.91% of pyrene could be removed within 10 d from an initial concentration of 50 mg·L-1. The percentage removed by the immobilized cells was 58% higher than that removed by free cells. In addition, the immobilized beads could be reused up to 9 times. |
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