磷镉交互作用对白骨壤幼苗体内镉的亚细胞分布和生理特性的影响

王岚; 戴闽玥; 严重玲

文章摘要

王岚,戴闽玥,严重玲.磷镉交互作用对白骨壤幼苗体内镉的亚细胞分布和生理特性的影响[J].农业环境科学学报,2018,37(4):640-646.

磷镉交互作用对白骨壤幼苗体内镉的亚细胞分布和生理特性的影响

Effects of phosphorus and cadmium interaction on cadmium subcellular distribution and physiological characteristics in Avicennia marina(Forsk.) Vierh seedlings

投稿时间：2017-09-01

DOI：10.11654/jaes.2017-1193

中文关键词: 磷镉白骨壤亚细胞分布生理特性

英文关键词: phosphorus cadmium Avicennia marina(Forsk.) Vierh subcellular distribution physiological characteristics

基金项目:国家自然科学基金重点项目（31530008）

作者	单位	E-mail
王岚	铜仁学院农林工程与规划学院, 贵州铜仁 554300
戴闽玥	厦门大学环境与生态学院, 福建厦门 361102
严重玲	厦门大学环境与生态学院, 福建厦门 361102	ycl@xmu.edu.cn

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中文摘要:

为研究镉（Cd）和磷（P）交互作用对白骨壤幼苗体内Cd亚细胞分布和生理特性的影响，以一年生白骨壤幼苗为试验材料，采用差速离心法提取不同亚细胞组分，氮蓝四唑法、愈创木酚法、分光光度法和考马斯亮蓝G-250法分别测定超氧化物歧化酶（SOD）、过氧化物酶（POD）活性和叶绿素、蛋白质含量。结果表明：随着Cd浓度的增加，白骨壤幼苗体内各亚细胞组分的Cd含量逐渐增加，其中细胞壁和可溶性组分中Cd含量增加较多，就分布而言，大部分的Cd（占整个植株的65.23%~96.92%）分布于白骨壤幼苗的根部。Cd集中分布于根中，表明细胞壁的固定及液泡区室化是白骨壤幼苗对Cd的解毒机制之一。研究也发现：外源P能促使Cd向液泡转移，同时提升了植株的抗氧化能力，表现为白骨壤植株SOD、POD活性增加、叶绿素和蛋白质含量升高。外源P能够缓解Cd对红树植物白骨壤的影响，增强白骨壤对Cd的耐受能力。

英文摘要:

A hydroponic experiment was carried out to study the effects of phosphorus and cadmium interaction on cadmium subcellular distribution and physiological characteristics in Avicennia marina(Forsk.) Vierh seedlings. Avicennia marina seedlings were used as the test materials. Four extraction methods; differential speed centrifugation, nitroblue tetrazolium, guaiacol, spectrophotometry and coomassie, were respectively adopted to determine the activity of SOD and POD, together with the chlorophyll and protein content. The results showed that increased Cd level in the medium caused a significant increase of Cd concentration in all fractions of leaves and roots, with most accumulation in the cell wall and soluble fraction. The content of Cd in roots was significantly higher than that in leaves, accounting for 65.23%~96.92%. Cellular compartmentation was one of the Cd detoxification mechanisms. Phosphorous could promote Cd transfer to the vacuole. With increasing P level, SOD, POD activities, chlorophyll and protein content also increased to alleviate the toxicity of Cd in A. marina seedlings to a certain extent, and thus increased the Cd tolerance of A. marina seedlings.

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