文章摘要
重金属复合污染土壤上南方红豆杉的根际细菌群落修复促生机制
Remediation and growth promotion mechanism of rhizosphere bacterial community of Taxus chinensis var. mairei on multi-metal contaminated soil
投稿时间:2019-03-29  修订日期:2019-04-30
DOI:
中文关键词: 南方红豆杉  重金属  土壤  经济价值  细菌群落
英文关键词: Taxus wallichiana  heavy metal  soil  economic value  bacterial community
基金项目:国家重点研发计划重点专项(2017YFD0801303),国家自然科学(41877116)
作者单位E-mail
魏帅 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室 weis@zju.edu.cn 
Wolde Tefera Beri 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室  
王润泽 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室  
林之 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室  
谢若瀚 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室  
乔亚蓓 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室  
卢玲丽 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室  
田生科 浙江大学环境与资源学院污染环境修复与生态健康教育部重点实验室 tiansk@zju.edu.cn 
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中文摘要:
      重金属污染土壤的经济利用是目前土地资源利用研究的热点问题。本文以高经济价值植物南方红豆杉(Taxus wallichiana var. mairei)为研究材料,在田间Cd/Cu复合污染土壤上开展大田试验,采用高通量测序和ICP-OES分析技术,研究了复合污染土壤条件下南方红豆杉根际细菌群落特征、特异细菌群落结构,测定了南方红豆杉各部位重金属的含量,以期明确复合重金属污染土壤对南方红豆杉价值的影响。研究结果表明:南方红豆杉能够显著改变重度复合污染土壤的细菌多样性,根际和非根际细菌群落结构差异显著。南方红豆杉根际能特异募集一些重金属活化菌及促生细菌,其中重金属活化菌可能通过分泌铁载体活化根际重金属,促进根系对镉铜的吸收,促生细菌则可能通过抵抗真菌入侵、改善植物根系对营养元素的吸收等方式实现其促生效果。与非根际土壤相比,南方红豆杉可以显著降低其根际土壤的Cd和Cu含量,分别降低18.8%和55.1%;且南方红豆杉高价值部位重金属Cd和Cu含量偏低,分别是根部的24.8%、30.7%,说明重度复合污染土壤没有降低南方红豆杉的经济价值。本研究揭示了在Cd/Cu复合胁迫下, 南方红豆杉根际土壤与非根际土壤微生物学特性,为在重金属污染土壤上红豆杉种植利用提供了重要的理论依据。
英文摘要:
      The economic utilization of heavy metal contaminated soil is a hot issue in the current research on land resource utilization. A field experiment was conducted on Cd/Cu multi-contaminated soil in the field in order to investigate the influence of multi-contaminated conditions on its economic value. 16S rRNA gene sequencing, combined with ICP-MS were applied to analyzed the rhizobacteria community structure and concentration of Cd/Cu in different tissues of Taxus wallichiana under the stress of multi-metal (Cd, Cu). Our results showed that the cultivation of Taxus wallichiana significantly changed the soil bacterial diversity, in which bacterial alpha diversity was significantly reduced in the rhizosphere. There is obvious difference of the bacterial community structure between the rhizosphere and bulk compartment. The rhizosphere can specifically enriched some bacteria with heavy metal activation and growth-promoting bacteria which could promote the growth of Taxus wallichiana and enhanced its tolerance to heavy metals. The total and available concentrations of Cd and Cu in rhizosphere were significantly reduced by 18.8% and 55.1% respectively after the planting of Taxus wallichiana. And the heavy metal concentration of the high value parts of Taxus wallichiana is relatively low. In this study, a new high-value plant was found for the full utilization of complex heavy metal contaminated soil, and its specific rhizosphere bacterial community structure was specific. The research revealed the microbial characteristics of rhizosphere of Taxus wallichiana under Cd/Cu complex stress, which provided a theoretical basis for its utilization in heavy metal contaminated soil.
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