| 夏丽丹,张虹,胡华英,曹升,周垂帆.RNA-Seq定量分析盐肤木对铅胁迫的响应[J].农业环境科学学报,2019,38(7):1459-1467. |
| RNA-Seq定量分析盐肤木对铅胁迫的响应 |
| Molecular responses of Rhus chinensis to lead stress revealed by RNA-Seq |
| 投稿时间：2018-12-11 |
| DOI：10.11654/jaes.2018-1571 |
| 中文关键词: 盐肤木 铅胁迫 核糖体 RNA-Seq |
| 英文关键词: Rhus chinensis lead stress ribosome RNA-Seq |
| 基金项目:福建省环保科技计划项目（2018R012）；福建农林大学科技创新专项基金项目（CXZX2018137，CXZX2018132） |
| 作者 | 单位 | E-mail | | 夏丽丹 | 福建农林大学林学院, 福州 350002
海峡两岸水土保持协同创新中心, 福州 350002
福建长汀红壤丘陵生态系统国家定位观测研究站, 福州 350002 | | | 张虹 | 福建农林大学林学院, 福州 350002
海峡两岸水土保持协同创新中心, 福州 350002
福建长汀红壤丘陵生态系统国家定位观测研究站, 福州 350002 | | | 胡华英 | 福建农林大学林学院, 福州 350002
海峡两岸水土保持协同创新中心, 福州 350002
福建长汀红壤丘陵生态系统国家定位观测研究站, 福州 350002 | | | 曹升 | 福建农林大学林学院, 福州 350002
海峡两岸水土保持协同创新中心, 福州 350002
福建长汀红壤丘陵生态系统国家定位观测研究站, 福州 350002 | | | 周垂帆 | 福建农林大学林学院, 福州 350002
海峡两岸水土保持协同创新中心, 福州 350002
1. 福建农林大学林学院, 福州 350002 | zhouchuifan@163.com |
|
| 摘要点击次数: 1903 |
| 全文下载次数: 1677 |
| 中文摘要: |
| 为探究重金属污染地生态修复先锋植物——盐肤木对重金属胁迫的分子响应机制，设置3个铅胁迫水平（0、250、1000mg·kg-1），应用Illumina HiSeqTM 2000进行盐肤木根系的转录组测序，并通过Gene Ontology（GO）与Pathway功能显著富集分析方法，分析说明盐肤木响应不同铅胁迫水平下的差异表达基因。结果表明，盐肤木在遭受轻度铅胁迫时，主要有4类水解酶与2类磷酸酶相关基因显著富集，说明盐肤木主要通过调节细胞内水解酶与磷酸酶相关基因来抵御胁迫，而在重度铅胁迫下，主要有6类氧化还原酶相关基因出现显著富集，此时氧化还原酶相关基因起到主要调节作用；盐肤木在铅胁迫下细胞受损，在轻、重度铅胁迫下，分别有24、16类细胞相关基因显著富集，但其自身可通过调节细胞内细胞器、细胞质等相关的细胞运动以应对逆境；核糖体代谢通路是盐肤木适应铅胁迫的主要代谢通路。研究表明，核糖体相关基因是盐肤木响应铅胁迫的主要应答与调节基因。 |
| 英文摘要: |
| Rhus chinensis is one of the pioneer plants for ecological restoration of heavy-metal-polluted areas. Since this species shows good tolerance to heavy metals such as lead and chromium, exploring its molecular response mechanism to heavy metal stress can provide a reference for the ecological restoration of heavy-metal pollution. In this study, we exposed R. chinensis to three concentrations of lead, corresponding to no, mild, and severe lead stress, respectively(0, 250, 1000 mg·kg-1), and performed transcriptome sequencing of the roots on the Illumina HiSeqTM 2000 system. Significant enrichment analysis was performed for Gene Ontology(GO)and Pathway functions, and the differentially expressed genes in response to the different lead concentrations were analyzed. Four types of hydrolases and two types of phosphatase-related genes were found to be differentially expressed in the mild lead-stress condition, which indicated that R. chinensis was mainly regulated by intracellular hydrolase and phosphatase-related genes. Under severe lead stress, six types of oxidoreductase-related genes were significantly enriched and played a major regulatory role. The cells were damaged under lead stress, and 24 and 16 cell-related genes were significantly enriched under mild and severe lead stress, respectively. However, R. chinensis could cope with this adversity by regulating cellular movements such as the movement of organelles and cytoplasm in cells. The ribosomal metabolic pathway was found to be the main metabolic pathway for salt tolerance and adaptation to lead stress, and ribosome-associated genes appeared to be the major regulatory genes induced in response to lead stress in R. chinensis. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
