| 李明珠,廖强,董远鹏,刘喜娟,孟子霖,李梦红,刘爱菊.铜胁迫下磺胺嘧啶对土壤呼吸及酶活性影响分析[J].农业环境科学学报,2019,38(9):2121-2128. |
| 铜胁迫下磺胺嘧啶对土壤呼吸及酶活性影响分析 |
| Effect of sulfadiazine on soil respiration and enzyme activity under copper stress |
| 投稿时间：2019-01-11 |
| DOI：10.11654/jaes.2019-0044 |
| 中文关键词: 磺胺嘧啶 铜 土壤酶活性 土壤呼吸 |
| 英文关键词: sulfadiazine Cu soil enzyme activity soil respiration |
| 基金项目:国家自然科学基金项目（41671322，41771348）；山东省自然科学基金项目（ZR2015DM010）；校城融合支持计划（2016ZBXC102） |
|
| 摘要点击次数: 1643 |
| 全文下载次数: 1570 |
| 中文摘要: |
| 为探究铜污染胁迫下磺胺嘧啶对土壤呼吸及酶活性的影响，本文选取环境中检出率较高的典型抗生素磺胺嘧啶（Sulfadiazine，SDZ）和重金属铜（Cu）为研究对象，设SDZ5（5 mg·kg-1）、SDZ10（10 mg·kg-1）及SDZ5+Cu200（5 mg·kg-1 SDZ+200 mg·kg-1 Cu）、SDZ5+Cu500（5 mg·kg-1 SDZ+500 mg·kg-1 Cu）、SDZ10+Cu200（10 mg·kg-1 SDZ+200 mg·kg-1 Cu）、SDZ10+Cu500（10 mg·kg-1 SDZ+500 mg·kg-1 Cu）6个处理，分别采用密闭法和酶试剂盒法测定SDZ单一及其与Cu复合污染处理下土壤呼吸作用及土壤酶活性的变化情况。结果表明，在试验设计浓度下，培养第7 d时，SDZ单一污染对土壤呼吸作用有明显的促进作用，而Cu的加入进一步促进了土壤的呼吸作用；但随着培养时间的延长，各污染处理的促进作用逐渐减弱，在培养第28 d时又恢复至对照水平。土壤脱氢酶对SDZ单一及其Cu复合污染较为敏感，在整个试验培养期内，各污染处理中其活性均受到了显著的抑制（P<0.05）。土壤磷酸酶对SDZ污染胁迫存在一定的耐受性和滞后性，其中，5 mg·kg-1 SDZ处理对土壤磷酸酶活性未产生显著的影响，10 mg·kg-1 SDZ仅在培养的第14、28 d对土壤磷酸酶产生显著的抑制作用；但SDZ和Cu复合污染对土壤磷酸酶则产生了显著的抑制作用。对于土壤脲酶，5 mg·kg-1 SDZ在培养前期无显著影响，培养14 d以后则表现显著的刺激作用；10 mg·kg-1 SDZ除在第7 d有明显刺激作用外对土壤脲酶并未产生显著影响，且200 mg·kg-1 Cu复合添加并未改变SDZ对土壤脲酶的影响，但其与500 mg·kg-1 Cu复合污染在培养7、14 d和28 d对土壤脲酶则产生了显著的抑制作用。5 mg·kg-1 SDZ对β-葡萄糖苷酶存在显著的刺激作用，但SDZ与500 mg·kg-1 Cu复合污染则对β-葡萄糖苷酶产生显著的抑制作用。本研究表明，土壤呼吸对SDZ单一及其与Cu复合污染胁迫响应与污染物浓度及暴露时间密切相关；不同土壤酶对SDZ单一及其与Cu复合污染的响应存在较大差异，土壤脲酶对各污染处理表现出较强的耐受性；但相比之下，脱氢酶对SDZ单一及与Cu复合污染较敏感，可以作为评价土壤SDZ单一及与Cu复合污染程度的敏感性指标，而土壤磷酸酶和β-葡萄糖苷酶则仅对SDZ与Cu复合污染较为敏感。 |
| 英文摘要: |
| In this study, sulfadiazine (SDZ)and copper (Cu)were chosen as typical pollutants to investigate their combined effects on soil microbial respiration and soil enzyme activities. The following six soil contamination treatments were established:SDZ5 (5 mg·kg-1), SDZ10 (10 mg·kg-1), SDZ5+Cu200 (5 mg·kg-1 SDZ and 200 mg·kg-1 Cu), SDZ5+Cu500 (5 mg·kg-1 SDZ and 500 mg·kg-1 Cu), SDZ10+Cu200 (10 mg·kg-1 SDZ and 200 mg·kg-1 Cu), and SDZ10+Cu500 (10 mg·kg-1 SDZ and 500 mg·kg-1 Cu). SDZ significantly promoted soil respiration at 7 days of incubation, and copper co-addition significantly strengthened this effect, which, however, gradually weakened and returned to the control level at the 28th day of incubation. Moreover, throughout the incubation period, soil dehydrogenase activity was strongly (P<0.05)inhibited by all of the contamination treatments, including SDZ single amendments and co-addition of Cu. Soil phosphatase activity showed a certain degree of tolerance and hysteresis to SDZ pollution, as there was no significant effect of 5 mg·kg-1 SDZ on soil phosphatase activity, and 10 mg·kg-1 SDZ induced significant inhibition only after 14~28 days of incubation; however, the combined pollution of SDZ and Cu resulted in significant (P<0.05)inhibition on soil phosphatase throughout the experiments. In contrast to dehydrogenase and phosphatase, urease activity was not significantly influenced by the treatments SDZ5 and SDZ5+Cu200, and only inhibited by treatment with SDZ10 on the 7th day of incubation and by treatment with SDZ along with 500 mg·kg-1 Cu at the 7th, 14th, and 28th day of incubation. Addition of 5 mg·kg-1 SDZ significantly stimulated β-G-glucosidase activity, which was greatly inhibited by treatment with SDZ and 500 mg·kg-1 Cu. Therefore, it could be concluded that the response of soil respiration to SDZ and its co-pollution with Cu was closely related to the concentration of the pollutants and exposure time. Further, soil enzymes showed different responses to SDZ and its co-pollution with Cu, which were greatly affected by the amounts of the pollutants. In contrast, dehydrogenase was more sensitive to SDZ single and Cu + SDZ combined pollution, which could be used as an indicator to evaluate these types of contaminants. However, soil phosphatase and β-Gglucosidase were only sensitive to the combined pollution with SDZ and heavy metals. |
| HTML
查看全文
查看/发表评论 下载PDF阅读器 |
|
|
|
