| 王真,张洪昌,沈根祥,胡双庆,钱晓雍,王振旗,李贞金.结合态雌激素在堆肥过程中的降解与转化研究[J].农业环境科学学报,2019,38(12):2860-2870. |
| 结合态雌激素在堆肥过程中的降解与转化研究 |
| Degradation and transformation of conjugated estrogens during composting |
| 投稿时间：2019-05-14 |
| DOI：10.11654/jaes.2019-0536 |
| 中文关键词: 堆肥 牛粪 结合态雌激素 降解 |
| 英文关键词: compost dairy feces conjugated estrogens degradation |
| 基金项目:国家自然科学基金青年科学基金项目（21607105）；水体污染控制与治理科技重大专项（2017ZX07207002） |
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| 中文摘要: |
| 为评价堆肥处理对牛粪中结合态雌激素的降解效果，本文采用微波萃取-固相萃取方法提取目标雌激素，利用高效液相色谱-串联质谱仪（HPLC-MS/MS）建立分析方法，研究了牛粪中15种雌激素类物质在堆肥周期70 d的实际堆肥过程中的降解与转化过程。结果显示：堆肥处理能有效降解牛粪中的E1-3G、E3-3G、OP、BPA、DES和EE2，而对NP的降解效率相对较低，堆肥结束后残留率仍为48.84%。其中，结合态雌激素在堆肥过程中首先水解为以E1为主的自由态，再进一步发生降解。堆肥70 d后E1浓度约为0 d的3倍。葡糖苷酸盐结合态的降解速率普遍高于硫酸盐结合态，降解率均≥ 98.76%，而硫酸盐结合态相对稳定，3种硫酸盐结合态在堆肥70 d仍能检测到，降解率在92.74%~96.79%。研究发现，最终可能通过有机肥还田过程进入到环境中的雌激素E1-3S、E2-3S、E2-17S、E2-3G、E2-17G、NP、E1和E2的浓度分别为1.75、9.5、16.4、3.89、4.44、233.4、69.28 μg·kg-1和11.98 μg·kg-1。研究表明，堆肥处理可作为畜禽粪便的处理方法，能有效削减粪便中雌激素含量，但直接还田存在一定的环境风险，需进一步对粪肥施用过程进行评价。 |
| 英文摘要: |
| A pretreatment method employing microwave and solid-phase extraction and an instrumental analytical method employing liquid chromatography-tandem mass spectrometry was developed to analyze the degradation and transformation of 15 target estrogens found in dairy feces undergoing a 70-day composting period. The results showed that E1-3G, E3-3G, OP, BPA, DES, and EE2 were degraded effectively during the composting, whereas NP degraded at a relatively slower rate, specifically at a 48.84% residual rate of NP. During composting, the conjugated estrogens were first hydrolyzed into free estrogens(mainly E1)and then degraded further. After 70 days of composting, the concentration of E1 was approximately three times its initial value. The degradation rates of glucuronide-conjugated estrogens were ≥ 98.76%, which was higher than those of sulfate-conjugated estrogens. The latter were relatively stable and could still be detected on day 70 at degradation rates between 92.74% and 96.79%. The study found that the estrogens, including E1-3S, E2-3S, E2-17S, E2-3G, E2-17G, NP, E1, and E2, might eventually enter the environment through fertilization at concentrations of 1.75, 9.5, 16.4, 3.89, 4.44, 233.4, 69.28, and 11.98 μg·kg-1, respectively. These results reveal that composting could be used as a treatment method for livestock manure, which can effectively reduce the estrogen content in feces. However, a certain amount of environmental risk remains after applying composted manure to a field, and further evaluation of manure application is needed. |
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