| 康恺,杨丹,黄至诚,张诗敏,方婷婷,江茵,吴江.微塑料对小鼠生长和小肠结构的影响[J].农业环境科学学报,2020,39(2):256-262. |
| 微塑料对小鼠生长和小肠结构的影响 |
| Effects of microplastics on the growth and structure of the mouse small intestine |
| 投稿时间：2019-08-02 |
| DOI：10.11654/jaes.2019-0851 |
| 中文关键词: 微塑料 小鼠 生长 小肠结构 |
| 英文关键词: microplastics mice growth small intestine structure |
| 基金项目:广东海洋大学博士启动基金（R17027，R17021）；广东省促进经济高质量发展专项资金海洋经济发展项目（GDOE2019A52） |
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| 中文摘要: |
| 为了探究微塑料对生物体的生长特性及肠道结构的影响，选取微塑料聚乙烯（PE）和聚苯乙烯（PS）作为原料，分别按0.3%（3 g·kg-1鼠粮）、3%（30 g·kg-1鼠粮）两种浓度混合添加于昆白小鼠日粮。饲喂期间记录小鼠的体重、饮食行为，27 d后进行剖检并采集小肠（十二指肠、空肠、回肠）制成石蜡切片观察显微结构。结果表明：对照组与微塑料添加组小鼠饮食与精神状态均正常，对照组小鼠体重在整个饲喂周期内逐渐增加，微塑料添加组在饲喂的前期体重增加，但在后续大部分小鼠体重增长停滞，甚至下降。不论是PE或PS微塑料添加组，整个小鼠体重增长均显著慢于对照组（P<0.01），并呈现浓度依赖性，但两种微塑料相比没有显著差异。肠道显微结构表明，微塑料添加组小鼠出现小肠绒毛肿胀、结构破坏、杯状细胞减少、黏膜下层增生等现象。小鼠摄入微塑料导致小肠显微结构破坏，生长减慢。 |
| 英文摘要: |
| To determine the effects of microplastics(MPs)on mouse growth and the intestinal structure, we selected the MPs polyethylene (MPs-PE)and polystyrene(MPs-PS)as raw materials, added respectively to Kunming white mouse chow at two concentrations:0.3%(3 g·kg-1 chow)and 3%(30 g·kg-1 chow). During feeding, we recorded the body weight and eating behavior of mice. After 27 days, mice were anatomized and the small intestine(duodenum, jejunum and ileum)was collected and used to make paraffin sections to observe the intestinal microstructure. Results showed that the diet and mental state of mice in both the control group and the MP addition group were normal. The body weight of the control group increased gradually during the feeding time, while the group with the MP additives showed an increased body weight in the early feeding times but stagnated and even declined in the later feeding times. The weight gain of the MP intake group was significantly slower than that in the control group(P<0.01), and showed a concentration dependence, though there was no significant difference in body weight between mice taking the two kinds of MPs. The intestinal microstructure showed that the small intestinal villi were swollen and structures were damaged, goblet cells were reduced, and there was hyperplasia of the submucosa. Thus, mice taking plastics show intestinal damage that lead to slower growth. |
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