| 吴晓斐,何源,黄治平,张丹丹,郑宏艳,丁健.不同处理梯度污水对细菌群落和酶活性的影响[J].农业环境科学学报,2020,39(9):2026-2035. |
| 不同处理梯度污水对细菌群落和酶活性的影响 |
| Effects of different treatment levels of sewage on bacterial community structure and enzyme activity |
| 投稿时间：2020-02-15 |
| DOI：10.11654/jaes.2020-0155 |
| 中文关键词: 污水 处理强度 灌溉 施肥水平 细菌 酶活性 |
| 英文关键词: wastewater treatment intensity irrigation fertilization level bacteria enzyme activity |
| 基金项目:国家水体污染控制与治理科技重大专项（2017ZX07603002） |
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| 摘要点击次数: 1664 |
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| 中文摘要: |
| 为寻求适合农村生活污水资源化利用的污水处理梯度，采用室内盆栽试验，设计生活污水原水、厌氧出水、接触氧化出水和尾水等4种污水处理梯度对茭白和水稻进行灌溉，研究不同处理梯度的生活污水对土壤细菌群落结构和酶活性的影响。结果表明：不同处理梯度的污水灌溉对土壤细菌群落多样性影响不显著，减少施肥有利于细菌群落多样性的增加；污水灌溉对土壤细菌门水平上的优势种影响较大，污水处理梯度与变形菌门具有正相关关系，与对照（清水）相比，土壤中放线菌门相对丰度提高16.74%~95.90%，酸杆菌门相对丰度降低9.57%~47.38%；污水灌溉对碱性磷酸酶和蔗糖酶活性具有促进作用，提高率为11.67%~55.60%和11.36%~156.66%；不施肥土壤中脲酶活性提高10.45%~25.88%，施肥土壤中接触氧化出水使脲酶活性降低25.84%，其他处理对施肥土壤脲酶活性无显著影响；在同一灌溉条件下，减少施肥有利于土壤酶活性提高。研究表明，肥料对土壤细菌群落结构和酶活性具有显著影响，不同处理梯度污水对土壤细菌多样性无显著性影响，但显著影响了土壤酶活性和细菌在门水平优势种的相对丰度。 |
| 英文摘要: |
| In order to determine a sewage treatment gradient suitable for the utilization of rural domestic sewage resources, indoor pot experiments was used to design four sewage treatment gradients including domestic sewage raw water, anaerobic effluent, contact oxidized effluent and tail water to irrigate Zizania latifolia and rice, and the effects of different treatment gradients of domestic sewage on the soil bacterial community structure and enzyme activity were studied. The results showed that sewage irrigation with different treatment gradients had no significant effect on the diversity of the soil bacterial communities, and reducing fertilization could increase the diversity of the bacterial communities. Sewage irrigation had a significant influence on the dominant species of soil bacteria at the phylum level. Compared with the control group, the gradient of sewage treatment had a positive correlation with Proteobacteria, the relative abundance of Actinomycetes in soil increased by 16.74% to 95.90%, and the relative abundance of Acidobacteria decreased by 9.57% to 47.38%. Sewage irrigation promoted the activity of alkaline phosphatase and sucrase, and the improvement rates were 11.67%~55.60% and 11.36%~156.66%, respectively. Urease activity in unfertilized soil increased by 10.45%~25.88%, and contact oxidized effluent reduced the urease activity by 25.84%. Other treatments had no significant effect on the urease activity in fertilized soil. Under the same irrigation condition, the activity of the three enzymes in the unfertilized soil was greater than that in the fertilized soil. The results showed that fertilizer had a significant effect on the soil bacterial community structure and enzyme activity. Different treatment gradients had no significant effect on the soil bacterial diversity, but significantly affected the soil enzyme activity and relative abundance of dominant species in each phylum. |
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