| 王鹤潼,贾春云,张延召,赵强,李晓军,巩宗强,刘宛.植物DNA错配修复系统响应Cd胁迫的研究进展[J].农业环境科学学报,2021,40(4):700-711. |
| 植物DNA错配修复系统响应Cd胁迫的研究进展 |
| Research progress on the response of DNA MMR system to cadmium stress in higher plants |
| 投稿时间：2020-10-02 |
| DOI：10.11654/jaes.2020-1152 |
| 中文关键词: Cd胁迫 DNA错配修复系统 植物 DNA损伤 细胞周期阻滞 |
| 英文关键词: Cd stress DNA mismatch repair(MMR) plants DNA lesions cell cycle arrest |
| 基金项目:国家自然科学基金项目（21677151，41807488，41673132） |
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| 摘要点击次数: 1991 |
| 全文下载次数: 1821 |
| 中文摘要: |
| Cd胁迫下植物细胞内会出现不同形式的DNA损伤（如碱基错配、DNA单/双链断裂和甲基化），DNA损伤会迅速诱导DNA损伤响应（DDR）信号，如ATM、ATR及其下游的信号通路包括细胞周期阻滞、细胞内复制、细胞凋亡以及不同形式的DNA修复途径（如同源重组修复HR、DNA错配修复MMR）。植物细胞DNA MMR系统中的异源二聚体MutSα（MSH2/MSH6）、MutSβ（MSH2/MSH3）、MutSγ（MSH2/MSH7）、MutLα（MLH1/PMS1）与有关酶如增殖细胞核抗原（PCNA）、DNA复制因子C（RFC）、DNA核酸外切酶1（EXO1）、单链结合蛋白（RPA）、核酸内切酶FEN1、DNA聚合酶delta（δ）和DNA连接酶相互作用，启动DNA MMR反应，从而在感知Cd诱导的DNA损伤、修复DNA损伤、激活细胞周期检验点、确保基因组DNA的稳定性和DNA复制的精确性等方面发挥关键作用。然而，MutS缺失的植物细胞会绕过DNA MMR系统参与的细胞周期阻滞，从而严重影响植物的耐Cd性能。本文重点介绍了植物DNA MMR系统对Cd胁迫的响应以及表观遗传对DNA MMR系统的调控作用机理。 |
| 英文摘要: |
| Cadmium(Cd) is one of the heavy metal pollutants with the most severe toxicity to bodies of water and agricultural soil in China. Various DNA lesions occur in plant cells under Cd stress, such as mismatched bases, DNA single/double strand breaks, and methylation. These DNA lesions rapidly induce DNA damage response(DDR) signaling, such as ataxia-telangiectasia mutated(ATM), ATM and RAD3-related(ATR), and their downstream signal pathway, including cell cycle arrest, endoreplication, cell death, and recruitment of DNA repair pathways, such as homologous repair(HR) and DNA mismatch repair(MMR) systems. In the DNA MMR system of plant cells, the heterodimeric complexes, including MutSα(MSH2/MSH6), MutSβ(MSH2/MSH3), MutSγ(MSH2/MSH7), and MutLα(MLH1/PMS1), can interact with key enzymes(e.g., proliferating cell nuclear antigen(PCNA), DNA replication factor C(RFC), exonuclease 1(EXO1), single-strand binding protein(RPA), flap endonuclease 1(FEN1), DNA polymerase delta(δ), and DNA ligase), participating in the DNA MMR system in order. The above-mentioned processes can initiate the DNA MMR response, which plays important roles in sensing DNA lesions, coping with DNA damage, activating the cell cycle checkpoints, maintaining genomic DNA stability, DNA replication fidelity, etc. However, plant cells with a MutS knockout(KO) override the cell cycle arrest regulated by the DNA MMR system, which can severely affect plant tolerance to Cd. In this review, we emphatically illustrate how the plant DNA MMR system works in a Cd-induced DDR and how multiple epigenetic factors regulate the DNA MMR system under Cd stress. |
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