文章摘要
刘东昀,袁永强,仇荣亮,王诗忠,黄雄飞,黄海燕.根际促生菌Enterobacter sp.EG16对小白菜生长及硒吸收的影响[J].农业环境科学学报,2021,40(7):1420-1431.
根际促生菌Enterobacter sp.EG16对小白菜生长及硒吸收的影响
Effect of plant growth promoting rhizobacteria Enterobacter sp.EG16 on the growth and Selenium uptake of Brassica chinensis L.
投稿时间:2020-10-21  
DOI:10.11654/jaes.2020-1213
中文关键词: 根际促生菌    小白菜  生物强化  生物富集
英文关键词: plant growth-prompting rhizobacteria(PGPR)  selenium (Se)  Brassica chinensis L.  biofortification  bioconcentration
基金项目:国家自然科学基金项目(41703073);高校基本科研业务费青年教师培育项目(18lgpy44);2019国家重点研发计划项目(2019YFC1805300)
作者单位E-mail
刘东昀 中山大学环境科学与工程学院, 广州 510006
广东省环境污染控制与修复技术重点实验室, 广州 510006 
 
袁永强 中山大学环境科学与工程学院, 广州 510006 yuanyq7@mail.sysu.edu.cn 
仇荣亮 中山大学环境科学与工程学院, 广州 510006
广东省环境污染控制与修复技术重点实验室, 广州 510006
广东省土壤重金属污染修复工程技术研究中心, 广州 510275
岭南现代农业科学与技术广东省实验室, 广州 510642 
 
王诗忠 中山大学环境科学与工程学院, 广州 510006
广东省环境污染控制与修复技术重点实验室, 广州 510006
广东省土壤重金属污染修复工程技术研究中心, 广州 510275 
wshizh2@mail.sysu.edu.cn 
黄雄飞 中山大学环境科学与工程学院, 广州 510006  
黄海燕 河南润友林业技术服务有限公司, 郑州 450008  
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中文摘要:
      为了对根际促生菌(Plant grow-prompting rhizobacteria,PGPR)与硒共同作用对植物生长的影响做进一步研究,通过水培实验,研究了不同浓度的外源硒条件下,接种根际促生菌Enterobacter sp.EG16对小白菜生长、生理代谢以及硒含量的影响。结果表明,无论接种EG16与否,硒对小白菜的生长均产生低促高抑的影响,开始产生生理毒害的阈值范围为5~10 mg·L-1。蛋白质、丙二醛(MDA)含量随硒浓度上升而增加,过氧化氢酶(CAT)活性则随硒浓度上升先增加后减少。单独接种EG16使小白菜的鲜质量和根分支数显著增加,并促使小白菜根系体积增大,还使得小白菜体内MDA含量显著降低。低浓度硒(≤5 mg·L-1)条件下,EG16的接种促使小白菜叶片数增加,并使根系伸长、根分支变多、根系体积增大。EG16也一定程度上促进了硒从小白菜地下部向地上部的转运。高浓度硒(≥10 mg·L-1)条件下,虽然小白菜地上部与地下部硒含量显著上升,但其受到较为严重的生长胁迫,小白菜部分死亡。而接种EG16的小白菜体内过氧化物酶(POD)活性显著上升,植物抗逆性有所增强,在高硒环境下存活率更高。研究表明,EG16能促进小白菜的生长,并在一定程度上促进小白菜的硒积累,在高硒胁迫环境中还能提高小白菜的抗逆性。
英文摘要:
      To study the interactions between selenium(Se)and plant growth-prompting rhizobacteria(PGPR)on plants, batch hydroponic experiments were conducted to analyze the interactions of exogenous Se and the inoculation with Enterobacter sp.EG16, a type of PGPR, on growth, physiological metabolism, and Se content of Brassica chinensis L. Results showed that lower Se concentrations were beneficial to the growth of Brassica chinensis L. whereas higher Se concentrations had negative effect whether or not the EG16 was applied. The threshold concentration of Se before it became toxic ranged from 5 to 10 mg·L-1. With the increase in the concentration of Se, the content of protein and malondialdehyde(MDA)increased, whereas the activities of catalase(CAT)enzymes increased at first and then decreased. The treatments in which plants were inoculated with EG16 solely increased the biomass, root volume, and the number of root branches but reduced MDA content significantly. At lower concentrations of Se(≤5 mg·L-1), inoculation with EG16 not only increased the number of leaves, root volume, and number of root branches but also promoted root elongation. EG16 promoted the transfer of Se from underground to above ground. At higher concentrations(≥10 mg·L-1)of Se, although the Se concentrations both above ground and underground increased significantly, the growth of the plants was significantly inhibited, and the survival rate declined. However, the plants inoculated with EG16 had high peroxidase(POD)activity and high survival rate, which indicated that their resistance ability might be enhanced. The results show that EG16 not only promote the growth and Se accumulation in Brassica chinensis L., but also improve the stress resistance of plants at higher concentrations of Se.
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