| 王思淇,张克强,孔德望,高文萱,韦红,梁军锋,李佳佳,杜连柱.微生物群落对厌氧干发酵挥发性脂肪酸抑制的响应[J].农业环境科学学报,2022,41(1):171-182. |
| 微生物群落对厌氧干发酵挥发性脂肪酸抑制的响应 |
| Microbial community response to volatile fatty acid inhibition in dry anaerobic fermentation |
| 投稿时间：2021-05-06 |
| DOI：10.11654/jaes.2021-0529 |
| 中文关键词: 厌氧干发酵 挥发性脂肪酸抑制 猪粪 秸秆 微生物群落 |
| 英文关键词: dry anaerobic fermentation volatile fatty acid inhibition pig manure straw microbial community |
| 基金项目:国家重点研发计划项目(2017YFD0800800); 中国农业科学院协同创新任务(CAAS-XTCX2016015); 中央级公益性科研院所基本科研业务费专项(2021-jbkyywf-dlz) |
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| 中文摘要: |
| 为研究挥发性脂肪酸（Volatile fatty acid，VFA）抑制对厌氧干发酵体系微生物的影响，在接种率25%[以挥发性固体（VS）计]条件下以猪粪（P组）、猪粪秸秆混合物（VS比1∶1，M组）为原料开展厌氧干发酵实验，结合体系中产气性能、理化指标，分析微生物群落对VFA抑制的响应。结果表明，两组发酵体系均出现VFA抑制，且M组抑制效应更明显，体现在日甲烷产量（以VS计）一直低于0.99 mL·g-1。高通量测序结果显示，VFA抑制下，2种发酵体系中细菌门以Firmicutes为主，主要优势菌属为Clostridium sensu stricto；优势古菌门为Euryarchaeota，氢营养型产甲烷菌Methanosphaerula和乙酸营养型产甲烷菌Methanothrix为主要优势菌属。混合发酵体系中以氢营养型产甲烷途径为主。2组发酵体系中产生的主要酸抑制种类不同，P组中酸抑制主体为丙酸，而M组中为乙酸。本实验中，猪粪秸秆混合原料比猪粪单独发酵的酸抑制作用更强，并且细菌和古菌协同作用受到阻碍，严重影响了体系的产气性能，酸抑制的发生对菌群结构的演替有很大影响。 |
| 英文摘要: |
| This study investigated the effect of volatile fatty acid(VFA)inhibition on microorganisms in a dry anaerobic fermentation system. Pig manure(group P)and a mixture of pig manure and straw(volatile solid ratio 1∶1, group M)were used as raw materials for this experiment. The microbial community response to VFA inhibition was analyzed by combining gas production performance and physical and chemical indicators. The results showed that both fermentation systems were significantly inhibited by VFA, where the inhibitory effect of group M was more obvious; this was reflected by its specific methane yield, which was < 0.99 mL·g-1. The results from high-throughput sequencing showed that under VFA inhibition, Firmicutes was the main bacterial phylum in the two fermentation systems, and the most abundant genus was Clostridium sensu stricto. Euryarchaeota dominated the archaeal community, whereas the hydrogenotrophic methanogen, Methanosphaerula, and the aceticlastic methanogen, Methanothrix, were the dominant genera. Hydrogenotrophic methanogenesis was the principle pathway for methane formation in the co-digestion system. The main acid inhibitors produced in the two systems differed; in group P it was propionic acid, whereas it was acetic acid for group M. In this experiment, the mixture of pig manure and straw had a stronger acid inhibitory effect than pig manure alone. Additionally, the synergistic effect of bacteria and archaea was hindered, seriously affecting the gas production performance of the system. The occurrence of acid suppression greatly influenced the succession of bacterial flora. |
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