| 钟俊杰,李晓镜,尹泽润,盛浩,聂三安.紫泥田水稻土细菌群落对不同农艺调控措施的响应[J].农业环境科学学报,2022,41(2):367-374. |
| 紫泥田水稻土细菌群落对不同农艺调控措施的响应 |
| Response of bacterial communities to different agronomic regulation measures in purple mud paddy fields |
| 投稿时间：2021-06-08 |
| DOI：10.11654/jaes.2021-0654 |
| 中文关键词: 石灰 有机肥 调理剂 细菌群落结构 高通量测序 |
| 英文关键词: lime organic fertilizer conditioner bacteria community structure high-throughput sequencing |
| 基金项目:湖南省农业委员会项目（农业部农办财函〔2015〕38号）；湖南省教育厅双一流学科优秀青年项目（19B282） |
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| 中文摘要: |
| 为研究土壤细菌群落对不同农艺措施的响应，揭示土壤细菌群落结构与土壤环境因子的相互关系，以紫泥田水稻土为研究对象，设置石灰、商品有机肥、土壤调理剂3个处理，对土壤细菌16S rRNA基因测序，分析不同处理对细菌群落组成和多样性的影响。结果表明：与对照相比，有机肥和调理剂处理提高了土壤细菌α多样性指数，而石灰处理降低了土壤细菌α多样性指数；石灰和有机肥处理的细菌α多样性显著高于调理剂处理。与对照相比，石灰、有机肥和调理剂处理酸杆菌门的相对丰度分别提高了64.77%、95.69%和67.52%，石灰处理变形菌门和硝化螺旋菌门的相对丰度分别提高了10.40%和11.99%，而有机肥处理降低了9.17%和43.06%，调理剂处理降低了10.67%和31.07%。主成分分析结果表明，与对照相比，石灰和有机肥处理土壤细菌群落结构较为相似，但调理剂处理改变了土壤细菌群落结构。冗余分析结果表明，土壤pH和全钾是影响土壤细菌群落结构的主要因子，阳离子交换量、全磷、有效态镉、全氮、微生物量碳和土壤有机碳影响较小。研究表明，不同农艺调控措施改变了紫泥田水稻土细菌的群落结构组成和多样性。 |
| 英文摘要: |
| This study was conducted to investigate the response of soil bacterial community structure characteristics to different agronomic measures, as well as the relationship between community structure and soil environmental factors. Three treatments including lime, commercial organic fertilizer, and soil conditioner were set in purple mud paddy fields. The 16S rRNA gene of soil bacteria was sequenced, and the effects of different treatments on bacterial community composition and diversity were analyzed. The results showed that different agronomic measures changed the composition and diversity of soil bacterial communities. Compared with the control, the organic fertilizer and conditioner treatments increased the bacterial α diversity index value in soil; however, the lime treatment decreased this value. The bacterial α diversity value after the lime and organic fertilizer treatment was significantly higher than that after the conditioner treatment. Compared with that in the control soil, the relative abundance of Acidobacteria increased by 64.77%, 95.69% and 67.52% in the lime-, organic fertilizer-, and conditioner-treated soils, respectively. The relative abundances of Proteobacteria and Nitrospira increased by 10.40% and 11.99% after the lime treatment, whereas they decreased by 9.17% and 43.06% after the organic fertilizer treatment and decreased by 10.67% and 31.07% after conditioner treatment, respectively. Principal component analysis results showed that the bacterial community structure of the lime-and organic fertilizer-treated soils was similar to that of the control soil. Conversely, conditioner treatment substantially changed the soil bacterial community structure. The redundancy analysis indicated that the bacterial community structure was mainly affected by soil pH and total potassium but was marginally affected by soil cation exchange capacity(CEC), total phosphorus(TP), available Cd, total nitrogen(TN), microbial biomass carbon(MBC), and soil organic carbon(SOC). The composition and diversity of bacterial community structure in purple mud paddy soils undergo changes as responses to different agronomic control measures, thus providing a theoretical basis for developing strategies to improve agricultural production function of purple mud paddy soils. |
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