| 陶雪莹,徐应明,王林,刘畅,孙约兵,梁学峰.叶面施锌对油菜镉锌生物可给性与形态的影响[J].农业环境科学学报,2022,41(4):735-745. |
| 叶面施锌对油菜镉锌生物可给性与形态的影响 |
| Effects of foliar zinc application on the bioaccessibilities and chemical forms of cadmium and zinc in Brassica chinensis L. |
| 投稿时间：2021-09-27 |
| DOI：10.11654/jaes.2021-1123 |
| 中文关键词: 油菜 叶面施锌 镉 生物可给性 化学形态 |
| 英文关键词: Brassica chinensis L. foliar zinc application cadmium bioaccessibility chemical form |
| 基金项目:天津市自然科学基金重点项目（18JCZDJC34000）；国家重点研发计划项目（2018YFD0800203）；国家自然科学基金项目（41571322） |
| 作者 | 单位 | E-mail | | 陶雪莹 | 农业农村部环境保护科研监测所, 天津 300191
农业农村部产地环境污染防控重点实验室, 天津 300191 | | | 徐应明 | 农业农村部环境保护科研监测所, 天津 300191
农业农村部产地环境污染防控重点实验室, 天津 300191 | | | 王林 | 农业农村部环境保护科研监测所, 天津 300191
农业农村部产地环境污染防控重点实验室, 天津 300191 | wanglin2017@caas.cn | | 刘畅 | 农业农村部环境保护科研监测所, 天津 300191
农业农村部产地环境污染防控重点实验室, 天津 300191 | | | 孙约兵 | 农业农村部环境保护科研监测所, 天津 300191
农业农村部产地环境污染防控重点实验室, 天津 300191 | | | 梁学峰 | 农业农村部环境保护科研监测所, 天津 300191
1. 农业农村部环境保护科研监测所, 天津 300191 | |
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| 中文摘要: |
| 为探究叶面施锌（Zn）对叶用油菜镉（Cd）和Zn生物可给性的影响与调控机理，通过盆栽试验，采用体外消化和连续提取方法，研究喷施不同浓度ZnSO4和EDTA·Na2Zn对两种油菜（Cd低积累品种华骏和普通品种寒绿）地上部Cd/Zn含量、生物可给性以及化学形态的影响，并通过相关性分析和线性拟合方法研究叶面施Zn影响下Cd/Zn生物可给性的变化与形态占比的关系。结果表明，叶面施Zn对油菜地上部Cd含量无显著影响，Zn含量显著升高。喷施ZnSO4降低了油菜Cd和Zn的生物可给性比例，最大降幅分别为24.37%和6.07%；而喷施4 mmol·L-1 EDTA·Na2Zn则显著提高了油菜生物可给态Cd含量，同时提高了Zn的生物可给性比例，最大增幅分别为49.19%和8.40%。喷施ZnSO4明显降低油菜乙醇提取态Zn的占比，最大降幅为10.88%；而喷施EDTA·Na2Zn则明显提高油菜乙醇、H2O提取态Cd以及乙醇提取态Zn的占比，最大增幅分别为25.59%、52.74%以及13.23%。油菜Cd/Zn的乙醇和H2O提取态含量与其生物可给态含量、乙醇和H2O提取态占比与其生物可给性比例分别呈极显著的正相关性，这表明油菜Cd/Zn的乙醇和H2O提取态对其生物可给性贡献最大，叶面施Zn主要通过影响乙醇和H2O提取态Cd/Zn进而调控其生物可给性。 |
| 英文摘要: |
| A pot experiment was conducted to evaluate the effect of foliar Zn application on the concentrations, bioaccessibilities, and chemical forms of Cd and Zn in Brassica chinensis L. and to reveal the underlying mechanisms. ZnSO4 and EDTA · Na2Zn at different concentrations were applied as foliar fertilizers. The bioaccessibilities of Cd and Zn were determined using the in vitro digestion method, and the chemical forms of Cd and Zn were measured using the continuous extraction method. The relationship between the bioaccessibilities of Cd and Zn and their chemical forms was investigated using correlation analysis and linear regression. The results showed that the foliar Zn application had an insignificant effect on the Cd concentrations in the aboveground parts of B. chinensis, but the Zn concentrations increased significantly. The foliar application of ZnSO4 reduced the bioaccessibility percentages of Cd and Zn in B. chinensis with maximal reductions of 24.37% and 6.07%, respectively, whereas the foliar application of 4 mmol · L-1 EDTA · Na2Zn significantly increased the bioaccessible Cd concentration and the bioaccessibility percentage of Zn in B. chinensis with maximal increases of 49.19% and 8.40%, respectively. The foliar application of ZnSO4 reduced the proportion of ethanol extractable Zn in B. chinensis with a maximal reduction of 10.88%, whereas the foliar application of EDTA · Na2Zn increased the proportions of ethanol and deionized water extractable Cd and the proportion of ethanol extractable Zn in B. chinensis, with maximal increases of 25.59%, 52.74%, and 13.23%, respectively. The bioaccessible Cd and Zn concentrations in B. chinensis were highly significant correlated with the concentrations of Cd and Zn extracted using ethanol and deionized water, and the bioaccessibili percentages of Cd and Zn showed highly significant correlations with the proportions of ethanol and deionized water extractable Cd and Zn. These results indicate that ethanol and deionized water extractable Cd and Zn in B. chinensis are the main contributors to the bioaccessibilities of Cd and Zn, and foliar Zn application affects the bioaccessibilities of Cd and Zn through its effect on ethanol and deionized water extractable Cd and Zn. |
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