文章摘要
侯佳玉,闫磊,程锦,曾紫君,张雅茹,鲁克嵩,姜存仓.L-天冬氨酸纳米钙促进油菜生长的机理机制[J].农业环境科学学报,2022,41(7):1408-1416.
L-天冬氨酸纳米钙促进油菜生长的机理机制
Mechanistic mechanism of calcium L-aspartate nanoparticles to promote the growth of rape
投稿时间:2021-12-06  
DOI:10.11654/jaes.2021-1414
中文关键词: L-天冬氨酸纳米钙  油菜  生长情况  生理特性
英文关键词: calcium L-aspartate nanoparticles  rape  growth  physiological characteristic
基金项目:国家自然科学基金项目(41271320);中央高校基本科研业务费专项资金项目(2017PY055)
作者单位E-mail
侯佳玉 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070  
闫磊 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070
华中农业大学园艺林学学院园艺植物生物学教育部重点实验室, 武汉 430070 
 
程锦 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070  
曾紫君 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070  
张雅茹 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070  
鲁克嵩 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070  
姜存仓 华中农业大学资源与环境学院微量元素研究中心, 武汉 430070
石河子大学农学院新疆生产建设兵团绿洲生态农业重点实验室, 新疆 石河子 832000 
jcc2000@mail.hzau.edu.cn 
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中文摘要:
      为揭示外源L-天冬氨酸纳米钙[Ca (L-asp)-NPs]对作物生长的影响,以油菜为供试材料,通过水培试验,研究了不同浓度(0、25、50、75、100 mg·L-1)的Ca (L-asp)-NPs和钙离子[1.18 g·L-1 Ca (NO3)2·4H2O]对植株生物量、根系形态、钙含量、叶绿素、光合参数、丙二醛和可溶性蛋白含量的影响。结果表明:较低浓度(25、50 mg·L-1)的Ca (L-asp)-NPs对油菜生长影响不显著,较高浓度(100 mg·L-1)的Ca (L-asp)-NPs会提高植株生物量(如株高和根长)。与不施钙相比,Ca (L-asp)-NPs施用会促进油菜根系的生长,增加叶绿素含量,提高叶片光合速率,100 mg·L-1 Ca (L-asp)-NPs处理下油菜总根长、总表面积和根尖数分别增加了76.08%、30.78%和1 107.31%;净光合速率、胞间CO2浓度、气孔导度和蒸腾速率则分别提高了295.31%、31.90%、1 158.03%和376.74%。另外,Ca (L-asp)-NPs的施用会降低叶片丙二醛含量,提高可溶性蛋白含量。研究表明,外源Ca (L-asp)-NPs可以作为纳米肥料为油菜补充钙营养,100 mg·L-1 Ca (L-asp)-NPs对油菜生长的促进效果最佳,油菜长势最接近于适宜钙水平。Ca (L-asp)-NPs具有作为新型纳米钙肥料在农业生产中推广的潜力。
英文摘要:
      An experiment was carried out in a green-house at Huazhong Agricultural University to reveal the effects of exogenous Laspartate nano-calcium[Ca (L-asp) - NPs] on the growth of rape (Brassica napus) grown hydroponically. We applied six treatments:different concentrations of Ca (L-asp)-NPs (0, 25, 50, 75, 100 mg·L-1) and ionic calcium[1.18 g·L-1 Ca (NO3) 2]. The dry and fresh weight, root morphological parameters, calcium content, chlorophyll, photosynthetic parameters, MDA, and soluble protein content were and improved the photosynthetic rate of leaves. Applying <100 mg·L-1 Ca (L-asp)-NPs, the total root length, total surface area, and apical number increased significantly by 76.08%, 30.78%, and 1 107.31%, respectively; net photosynthetic rate, intercellular CO2 concentration, stomatal conductance, and transpiration rate also increased significantly by 295.31%, 31.90%, 1 158.03%, and 376.74%, respectively. In addition, the application of Ca (L-asp)-NPs decreased MDA levels in rape leaves, while increasing the content of soluble protein in the plants. The research shows that exogenous Ca (L-asp)-NPs can be used as a nano fertilizer to supplement calcium nutrition for rape. The optimum concentration of Ca (L-asp)-NPs is 100 mg·L-1 as this showed the best growth potential, while the growth of rape treated with <100 mg·L-1 Ca (L-asp)-NPs has the closest to the appropriate calcium level. Ca (L-asp)-NPs has the potential to be popularized as a new nano calcium fertilizer in agricultural production. determined. The results showed that lower concentrations of Ca (L-asp)-NPs (25, 50, 75 mg·L-1) had no significant effect on the growth of rape but a higher concentration of Ca (L-asp) - NPs (100 mg · L-1) increased plant biomass, including plant height and root length. Compared with NPS-0, the application of Ca (L-asp)-NPs promoted the growth of rape roots, enhanced the chlorophyll content of leaves, and improved the photosynthetic rate of leaves. Applying <100 mg·L-1 Ca(L-asp)-NPs, the total root length, total surface area, and apical number increased significantly by 76.08%, 30.78%, and 1 107.31%, respectively; net photosynthetic rate, intercellular CO2 concentration, stomatal conductance, and transpiration rate also increased significantly by 295.31%, 31.90%, 1 158.03%, and 376.74%, respectively. In addition, the application of Ca(L-asp)-NPs decreased MDA levels in rape leaves, while increasing the content of soluble protein in the plants. The research shows that exogenous Ca(L-asp)-NPs can be used as a nano fertilizer to supplement calcium nutrition for rape. The optimum concentration of Ca(L-asp)-NPs is 100 mg·L-1 as this showed the best growth potential, while the growth of rape treated with <100 mg·L-1 Ca(L-asp)-NPs has the closest to the appropriate calcium level. Ca(L-asp)-NPs has the potential to be popularized as a new nano calcium fertilizer in agricultural production.
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