| 孙晶,李伟,丛瑞,胡献刚,欧阳少虎.氧化石墨烯和多环芳烃复合暴露诱导成年斑马鱼脑组织毒性及其代谢研究[J].农业环境科学学报,2022,41(7):1417-1423. |
| 氧化石墨烯和多环芳烃复合暴露诱导成年斑马鱼脑组织毒性及其代谢研究 |
| Toxicity and metabolism of adult zebrafish brain tissue induced by combined exposure to graphene oxide and polycyclic aromatic hydrocarbons |
| 投稿时间：2021-10-27 |
| DOI：10.11654/jaes.2021-1228 |
| 中文关键词: 氧化石墨烯 多环芳烃 斑马鱼 复合暴露 代谢组学 |
| 英文关键词: graphene oxide polycyclic aromatic hydrocarbons zebrafish compound exposure metabolomes |
| 基金项目:国家重点研发计划项目(2019YFC1804104);博士后面上项目(2020M680867);NSFC山东联合基金资助项目(U1906222);高等学校学科创新引智计划项目(T2017002) |
| 作者 | 单位 | E-mail | | 孙晶 | 生态环境部海河流域北海海域生态环境监督管理局生态环境监测与科学研究中心, 天津 300061
南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津市城市生态环境修复与污染防治重点实验室, 天津 300071 | | | 李伟 | 生态环境部海河流域北海海域生态环境监督管理局生态环境监测与科学研究中心, 天津 300061 | | | 丛瑞 | 生态环境部海河流域北海海域生态环境监督管理局生态环境监测与科学研究中心, 天津 300061 | | | 胡献刚 | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津市城市生态环境修复与污染防治重点实验室, 天津 300071 | | | 欧阳少虎 | 南开大学环境科学与工程学院, 环境污染过程与基准教育部重点实验室, 天津市城市生态环境修复与污染防治重点实验室, 天津 300071 | ouyangshaohu@nankai.edu.cn |
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| 中文摘要: |
| 为研究氧化石墨烯(Grapheneoxide,GO)和多环芳烃(Polycyclicaromatic hydrocarbons,PAHs)共暴露诱发的水生生态毒性效应及相关代谢机理,使用成年斑马鱼为动物模型,研究不同浓度GO (0.01、0.1 mg·L-1)、PAHs (含16种优控PAHs,每种PAHs的浓度均为5 μg·L-1)单独暴露,以及相应浓度的GO-PAHs复合暴露21 d对成年斑马鱼脑组织的毒性及代谢的影响。斑马鱼脑组织酶响应结果表明,0.1 mg·L-1 GO和0.1 mg·L-1 GO-PAHs处理会显著(P<0.05)降低多环芳烃受体活性,除PAHs组外,其他处理组细胞色素P4501A1(CYP1A1)酶的含量均显著降低。代谢组学分析结果表明,0.1mg·L-1 GO组和0.1 mg·L-1 GO-PAHs复合暴露组均会对斑马鱼脑组织的氨基酸和脂肪酸代谢产生显著影响。此外,与GO和PAHs单独暴露组相比,GO-PAHs复合暴露组的酶响应和诱导产生的代谢物水平与GO处理组更相近,说明GO和PAHs复合暴露时,GO在成年斑马鱼脑组织中的代谢毒性效应占主导。 |
| 英文摘要: |
| With the increase of application and research on graphene oxide (GO), the risk possibility of its release into the environment has increased, where it may interact with polycyclic aromatic hydrocarbons (PAHs). However, the effects of GO and PAH co-exposure on aquatic ecotoxicity and related metabolic mechanisms are still largely unknown. In this work, adult zebrafish was chosen as a model organism to investigate the toxic effects of single exposure to different concentrations of GO (0.01 mg · L-1 and 0.1 mg · L-1) and PAHs (including 16 kinds of optimal control PAHs, each with a concentration of 5 μg · L-1), and combined exposure to GO-PAHs at the corresponding concentration for 21 days on adult zebrafish brain tissues and the metabolism in these tissues. Enzyme results showed that 0.1 mg·L-1 GO and 0.1 mg·L-1 GO-PAHs could significantly (P<0.05) decrease aromatic hydrocarbon receptor activity, and that all the treatments (except for PAHs) decreased concentration of cytochrome P4501A1 in adult zebrafish brain tissue. Metabolomics analysis results showed that 0.1 mg·L-1 GO, and 0.1 mg·L-1 GO-PAHs exposure groups significantly influenced amino acids and fatty acids levels in adult zebrafish brain tissue. Moreover, when compared with the GO and PAHs exposure groups, the enzyme response and metabolite levels induced by the GO-PAHS combined exposure were found to be close to those induced by the GO treatment, revealing that GO was dominant in inducing metabolic toxicity in brain tissue during combined exposure of GO and PAHs in adult zebrafish. |
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