| 叶彬,周涛,廉杰,李祎飞,邹华.湿地植物根系分泌物中有机酸对NSAIDs胁迫的响应[J].农业环境科学学报,2022,41(7):1555-1564. |
| 湿地植物根系分泌物中有机酸对NSAIDs胁迫的响应 |
| Organic acid responses in root exudates of wetland plant due to the stress of non-steroidal anti-inflammatory drugs |
| 投稿时间：2021-12-13 |
| DOI：10.11654/jaes.2021-1440 |
| 中文关键词: 根系分泌物 有机酸 非甾体类消炎药(NSAIDs) 湿地植物 高效液相色谱(HPLC) |
| 英文关键词: root exudate organic acid non-steroidal anti-inflammatorys drugs (NSAIDs) wetland plant high-performance liquid chromatography (HPLC) |
| 基金项目:江苏省自然科学基金项目(BK20180632);国家自然科学基金项目(42107418) |
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| 中文摘要: |
| 以人工湿地典型植物芦苇(Phragmites australis)为研究对象,探索芦苇根系分泌物中有机酸对非甾体类消炎药(NSAIDs)混合物(双氯芬酸、布洛芬、吲哚美辛、酮洛芬、萘普生)长期胁迫的响应。首先,优化测定有机酸的高效液相色谱(HPLC)法;然后,构建水培和人工湿地两种体系,于NSAIDs胁迫下(地表水浓度级别,100 ng·L-1),在芦苇幼苗期、展叶期和快速生长期,监测植物的生长生理情况,测定根部累积的NSAIDs浓度,并分析根系分泌物中有机酸的组分变化。结果表明:优化的HPLC方法可以在10 min内分离12种有机酸(草酸、酒石酸、奎尼酸、苹果酸、丙二酸、乳酸、乙酸、柠檬酸、琥珀酸、丙酸、富马酸和反乌头酸),检测限低于1 mg·L-1。在两种体系中均发现,随着植物的发育,根系释放速率增大的有机酸包括奎尼酸、柠檬酸、丙酸、丙二酸、苹果酸和草酸,且在快速生长期尤为明显,表明芦苇在NSAIDs胁迫下可能通过根系释放特定的有机酸促进根际微生物降解NSAIDs,从而减少根系对NSAIDs的吸收,人工湿地体系中芦苇根部NSAIDs累积量的降低和叶绿素抑制作用的减轻间接支持了这一猜测。研究表明,奎尼酸、柠檬酸、丙酸、丙二酸、苹果酸和草酸具备提高人工湿地处理地表水NSAIDs效率的潜力。 |
| 英文摘要: |
| This study investigated the organic acid responses in the root exudates of a typical wetland plant, Phragmites australis, to the long-term stress of a mixture of non-steroidal anti-inflammatory drugs (NSAIDs) :diclofenac, ibuprofen, indomethacin, ketoprofen, and naproxen. First, high-performance liquid chromatography (HPLC) was optimized for the analysis of organic acids. Then, a hydroponic system and a constructed wetland system were built up under the stress of NSAIDs (surface water level, 100 ng·L-1), in which Phragmites australis plants were cultivated at seedling, vegetative, and bolting stages. The organic acids in root exudates, growth and physiological indices of plants, and concentrations of NSAIDs accumulated in plant roots were analyzed for the two systems. The experimental results showed that the optimized HPLC method could determine 12 organic acids (oxalate, tartaric acid, quinate, malate, malonate, lactate, acetate, citric acid, succinic acid, propionic acid, fumarate, and rrans-aconitic acid) within 10 min, and the detection limits were all lower than 1 mg·L-1. In both systems, with plant development, the root secretion rates of quinate, citrate, propionic acid, malonate, malate, and oxalate increased when exposed to NSAIDs, especially at the bolting stage. This indicates that Phragmites australis in the constructed wetland system was prone to promote the microbial degradation of NSAIDs via the root release of certain organic acids, thus reducing the amount of NSAIDs absorbed by roots. The reduction in NSAIDs accumulation in roots and the inhibition of chlorophyll indirectly supported this deduction. Overall, quinate, citrate, propionic acid, malonate, malate, and oxalate showed the potential to enhance the efficiency of NSAID removal from surface water by constructed wetlands. |
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